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M-CSF Recombinant antibody, PBS Only

M-CSF Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241743C5

Cat no : 84481-4-PBS

Synonyms

M CSF, Lanimostim, CSF-1, CSF1, CSF 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using M-CSF Recombinant antibody (84481-4-RR)

    WB analysis using 84481-4-RR (same clone as 84481-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84481-4-RR (MCSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84481-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84481-4-RR

    Affinity and Kinetic Characterization of 84481-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84481-4-RR against Human M-CSF were performed. The affinity constant is 11.4 nM.

Product Information

84481-4-PBS targets M-CSF in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name colony stimulating factor 1 (macrophage)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC021117
Gene Symbol M-CSF
Gene ID (NCBI) 1435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)