HumanKine® recombinant human M-CSF protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.7-4.0 ng/mL

Specific Activity

minimally 4.00 x 10⁷ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

32

Cat no : HZ-1192

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

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Validation Data Gallery

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  Purity of recombinant human M-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human M-CSF (Cat no: HZ-1192) stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. The EC50 range is 0.7-4.0 ng/mL.

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  Proteintech M-CSF (Cat no: HZ-1192) demonstrates equivalent fold induction and a 2-fold better EC50 compared to leading competitors. Recombinant human M-CSF stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. The EC50 range is 0.7-4.0 ng/mL.

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Technical Specifications

• GeneID: 1435
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.7-4.0 ng/mL
• Specific Activity: minimally 4.00 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P09603
• Molecular Mass: 19 and 23 kDa reduced, 35 and 40 kDa non-reduced, homodimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

Publications

Species	Title
human	Cell Metab; Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK; Authors - Yajuan Li
mouse	Cell Metab; Senescent immune cells release grancalcin to promote skeletal aging.; Authors - Chang-Jun Li
mouse	Cell Metab; A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.; Authors - Hui Peng
mouse	ACS Nano; Bioinspired Nanovesicles Convert the Skeletal Endothelium-Associated Secretory Phenotype to Treat Osteoporosis.; Authors - Yongzhi Cui
mouse	Adv Sci (Weinh); Exosomal miR-181d-5p Derived from Rapamycin-Conditioned MDSC Alleviated Allograft Rejection by Targeting KLF6; Authors - Chao Wei
human	Autophagy; Induction of a Na+/K+-ATPase-dependent form of autophagy triggers preferential cell death of human immunodeficiency virus type-1-infected macrophages.; Authors - Gang Zhang