- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human M-CSF protein
Activity(Primary)
0.7-4.0 ng/mL
Specific Activity
minimally 4.00 x 107 IU/mg
Species Reactivity
human,mouse,rat
Purity
>95%
Cat no : HZ-1192
Synonyms
CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF
Validation Data Gallery
Technical Specifications
GeneID | 1435 |
Species | Human |
Expression | HEK293 |
Activity(Primary) | 0.7-4.0 ng/mL |
Specific Activity | minimally 4.00 x 107 IU/mg |
Purity | >95% |
Endotoxin | <1 EU/µg |
Accession Number | P09603 |
Molecular Mass | 19 and 23 kDa reduced, 35 and 40 kDa non-reduced, homodimer, glycosylated |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human,mouse,rat |
Stability and Reconstitution
Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
---|---|---|---|
Lyophilized | -20°C to -80°C | Until Expiry Date | |
Lyophilized | Room Temperature | 2 weeks | |
Reconstituted as per CofA | -20°C to -80°C | 6 months | |
Reconstituted as per CofA | 4°C | 1 week | |
Avoid repeated freeze-thaw cycles. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)
Synonyms
CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF
Publications
Species | Title |
---|---|
Cell Metab Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK | |
Cell Metab A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis. | |
ACS Nano Bioinspired Nanovesicles Convert the Skeletal Endothelium-Associated Secretory Phenotype to Treat Osteoporosis. | |
Adv Sci (Weinh) Exosomal miR-181d-5p Derived from Rapamycin-Conditioned MDSC Alleviated Allograft Rejection by Targeting KLF6 | |
Autophagy Induction of a Na+/K+-ATPase-dependent form of autophagy triggers preferential cell death of human immunodeficiency virus type-1-infected macrophages. |