HumanKine® recombinant human M-CSF protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

0.7-4.0 ng/mL

Specific Activity

minimally 4.00 x 10⁷ IU/mg, typically 5.45 x 10⁷ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1192-GMP

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

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Validation Data Gallery

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  Purity of recombinant human M-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  GMP recombinant human M-CSF ( Cat no: HZ-1192-GMP) stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.7-4.0 ng/mL.

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Technical Specifications

• GeneID: 1435
• Species: Human
• Expression: HEK293
• EC50: 0.7-4.0 ng/mL
• Specific Activity: minimally 4.00 x 10^7 IU/mg, typically 5.45 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <0.1 EU/µg
• Accession Number: P09603
• Molecular Mass: 19 and 23 kDa reduced, 35 and 40 kDa non-reduced, homodimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF