NeutraKine®M-CSF Monoclonal antibody, PBS Only (Capture)

NeutraKine®M-CSF Monoclonal Antibody for ELISA, Sandwich ELISA, Neutralization, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA, Sandwich ELISA, Neutralization, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C6C5

Cat no : 69033-1-PBS

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF, NeutraKine®M-CSF

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP50033-1

  Sandwich ELISA standard curve of MP50033-1, MCSF Monoclonal Matched Antibody Pair - PBS Only. 69033-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1192. 68662-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Neutralization experiment of NeutraKine®M-CSF using 69033-1-Ig (same clone as 69033-1-PBS)

  Recombinant human M-CSF(Cat.NO. HZ-1192) stimulates the proliferation of OCI-AML5 cells in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human M-CSF (5 ng/mL) is neutralized by mouse anti-human M-CSF monoclonal antibody 69033-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.1-0.4 μg/mL. The control mouse anti-human M-CSF monoclonal antibody 69533-1-Ig could recognize human M-CSF, but could not neutralize human M-CSF at serial does at the range of 0.976563-16000ng/mL(green curve, refer to top X-right Y). This data was developed using the same antibody clone with 69033-1-PBS in a different storage buffer formulation.

• 

  Neutralization experiment of NeutraKine®M-CSF using 69033-1-Ig (same clone as 69033-1-PBS)

  Recombinant human M-CSF (Cat.NO. HZ-1192) stimulates proliferation of OCI-AML5 cells in a dose dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human M-CSF (5ng/mL HZ-1192) is neutralized by mouse anti-human M-CSF monoclonal antibody 69033-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 0.1-0.4 μg/mL. This data was developed using the same antibody clone with 69033-1-PBS in a different storage buffer formulation.

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  ELISA experiment of Recombinant protein using 69033-1-Ig (same clone as 69033-1-PBS)

  Indirect ELISA was carried out by coating recombinant Human M-CSF (Cat.NO. HZ-1192) at 70 ng/well followed by blocking and adding serial diluted M-CSF antibody 69033-1-Ig and 69533-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69033-1-PBS in a different storage buffer formulation.

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Product Information

69033-1-PBS targets NeutraKine®M-CSF as part of a matched antibody pair:

MP50033-1: 69033-1-PBS capture and 68662-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=8.48 x 10^-9M
  K_Off=4.75 x 10^-4M
  K_On=5.60 x 10^4M
• Immunogen: human HumankineM-CSF protein HZ-1192
• Full Name: colony stimulating factor 1 (macrophage)
• Gene Symbol: M-CSF
• Gene ID (NCBI): 1435
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

M-CSF plays an essential role in the regulation of survival, proliferation, and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates the formation of membrane ruffles, cell adhesion, and cell migration, and plays a role in lipoprotein clearance.

This antibody is used to neutralize the bioactivity of M-CSF.