HRP-conjugated Heavy chain of Rabbit IgG Monoclonal antibody

Heavy chain of Rabbit IgG Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

rabbit and More (1)

Applications

WB, IP

Conjugate

HRP

Publications

4

CloneNo.

1C9A5

Cat no : HRP-66467

Synonyms

Heavy Chain, Heavy Chain of Rabbit IgG, Rabbit IgG

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Validation Data Gallery

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  WB analysis using HRP-66467

  Various serum/plasma lysate were subjected to SDS PAGE followed by western blot with HRP-66467 (Heavy chain of Rabbit IgG antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using HRP-66467

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. HRP-66467 (Heavy chain of Rabbit IgG antibody) as secondary antibody with dilution 1:15000. .

• 

  WB analysis of HeLa using HRP-66467

  HeLa cells were subjected to SDS PAGE followed by western blot with HRP-66467 (Heavy chain of Rabbit IgG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, rabbit serum tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IP: See 1 publications below

Product Information

HRP-66467 targets Heavy chain of Rabbit IgG in WB, IP applications and shows reactivity with rabbit samples.

• Tested Reactivity: rabbit
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Protein
• Full Name: Heavy chain of Rabbit IgG
• RRID: AB_2883840
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This antibody specifically recognizes the heavy chain of Rabbit IgG. It can't detect the The heavy chain of other species. This antibody can be used as the secondary antibody which is specific to the heavy chain of Rabbit IgG.

Publications

Species	Application	Title
	WB	Pharm Biol; Effects of total coumarins from Pileostegia tomentella on exosomal miRNA expression and angiogenesis in colorectal cancer cells; Authors - Ying Liu
	WB	Curr Med Sci; USP19 Stabilizes TAK1 to Regulate High Glucose/Free Fatty Acid-induced Dysfunction in HK-2 Cells; Authors - Xiao-Hui Yan
	WB	Transl Res; A key role for NLRP3 signaling in preterm labor and birth driven by the alarmin S100B; Authors - Jose Galaz
mouse	IP	FEBS J; NDRG2 alleviates photoreceptor apoptosis by regulating the STAT3/TIMP3/MMP pathway in mice with retinal degenerative disease; Authors - Tao Chen