Biotin-conjugated Heavy chain of Rabbit IgG Monoclonal antibody

Heavy chain of Rabbit IgG Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

rabbit

Applications

IHC

Conjugate

Biotin

Publications

2

CloneNo.

1C9A5

Cat no : Biotin-66467

Synonyms

Heavy chain of Rabbit IgG, Rabbit IgG

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Validation Data Gallery

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  IHC staining of human ovary tumor using Biotin-66467

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using PAX8 (10336-1-AP, 1:2000) as primary antibody, Biotin-66467 (Heavy chain of Rabbit IgG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using Biotin-66467

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using PAX8 (10336-1-AP, 1:2000) as primary antibody, Biotin-66467 (Heavy chain of Rabbit IgG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66467 targets Heavy chain of Rabbit IgG in IHC applications and shows reactivity with rabbit samples.

• Tested Reactivity: rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Protein
• Full Name: Heavy chain of Rabbit IgG
• RRID: AB_2883072
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody specifically recognizes the heavy chain of Rabbit IgG.

Publications

Species	Application	Title
		Exp Ther Med; CLCA2 suppresses the proliferation, migration and invasion of cervical cancer.; Authors - Peijin Zhang
		Cancer Biol Ther; EGR1-induced upregulation of lncRNA FOXD2-AS1 promotes the progression of hepatocellular carcinoma via epigenetically silencing DKK1 and activating Wnt/β-catenin signaling pathway.; Authors - Ting Lei