Heavy chain of Rabbit IgG Monoclonal antibody

• 

  WB analysis of rabbit serum using 66467-1-Ig

  rabbit serum (10nL) were subjected to SDS PAGE followed by western blot with 66467-1-Ig (mouse McAb to Heavy chain of Rabbit IgG) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Heavy chain of Rabbit IgG Monoclonal Antibody for WB, ELISA

Cat No. 66467-1-Ig

Clone No.1C9A5

Publications(6)

Host / Isotype

Mouse / IgG1

Reactivity

rabbit and More (1)

Applications

WB, IF, IP, CoIP, ELISA

Synonyms

Rabbit IgG

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Tested Applications

• Positive WB detected in: rabbit serum tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

66467-1-Ig targets Heavy chain of Rabbit IgG in WB, IF, IP, CoIP, ELISA applications and shows reactivity with rabbit samples.

• Tested Reactivity: rabbit
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Protein
• Full Name: Heavy chain of Rabbit IgG
• Observed Molecular Weight: 50-55 kDa
• RRID: AB_2881835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: N/A
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody specifically recognizes the heavy chain of Rabbit IgG.

Publications

Species	Application	Title
	IP	Adv Sci (Weinh); KIF11 UFMylation Maintains Photoreceptor Cilium Integrity and Retinal Homeostasis; Authors - Jie Ran
		Pharmacol Res; RHBDF1 deficiency suppresses melanoma glycolysis and enhances efficacy of immunotherapy by facilitating glucose-6-phosphate isomerase degradation via TRIM32; Authors - Lei Wang
	IF	Genomics; Effectiveness of a novel rat model of off-target PLA2R1 knockout to renal impairment; Authors - Bo Huang
		Oncol Lett; Long non-coding RNA RNCR3 promotes glioma progression involving the Akt/GSK-3β pathway.; Authors - Bing Zhu
	CoIP	Sci Total Environ; ACSL4-mediated ZIP7-VDAC3 interaction regulates endoplasmic reticulum-mitochondria iron transfer in hepatocytes under PFOS exposure; Authors - Peiyao Liang
rat	IF	Cell Biochem Funct; Effectiveness of a Novel PLA2R1 Knock-in Middle Age Rat Model in Repairing Renal Function Damage; Authors - Daihe Yang