CoraLite®594-conjugated Heavy chain of Rabbit IgG Monoclonal antibody

Heavy chain of Rabbit IgG Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

rabbit and More (1)

Applications

IHC, IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Publications

10

CloneNo.

1C9A5

Cat no : CL594-66467

Synonyms

Rabbit IgG

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66467

  Immunofluorescent analysis of (-20°C Acetone) fixed HeLa cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and CL594-66467 (CL594-Mouse anti-Rabbit IgG heavy chain) as secondary antibody with dilution 1:400. .

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  IF Staining of HepG2 using CL594-66467

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Heavy chain of Rabbit IgG antibody (CL594-66467, Clone: 1C9A5 ) at dilution of 1:500.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 7 publications below

Product Information

CL594-66467 targets Heavy chain of Rabbit IgG in IHC, IF/ICC applications and shows reactivity with rabbit samples.

• Tested Reactivity: rabbit
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Protein
• Full Name: Heavy chain of Rabbit IgG
• RRID: AB_2883585
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

This antibody specifically recognizes the heavy chain of Rabbit IgG. It can't detect the The heavy chain of other species. This antibody can be used as the secondary antibody which is specific to the heavy chain of Rabbit IgG. This anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Publications

Species	Application	Title
human	IF	Small; Microbial Lipopeptide Supramolecular Self-Assemblies as a Methuosis-Like Cell Death Inducer with In Vivo Antitumor Activity.; Authors - Jiao Yang Lu
		Nucleic Acids Res; MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis.; Authors - Yongliang Shang
	IF	J Cell Physiol; Transforming growth factor-β signaling confers hepatic stellate cells progenitor features after partial hepatectomy.; Authors - Zixin Chen
	IF	Hepatol Commun; TATA-box-binding protein promotes hepatocellular carcinoma metastasis through epithelial-mesenchymal transition; Authors - Jiayi Cao
		FASEB J; Senolytic therapy ameliorates renal fibrosis postacute kidney injury by alleviating renal senescence.; Authors - Caizhen Li
	IF	Ecotoxicol Environ Saf; Prenatal di-(2-ethylhexyl) phthalate exposure induced myocardial cytotoxicity via the regulation of the NRG1-dependent ErbB2/ErbB4-PI3K/AKT signaling pathway in fetal mice.; Authors - Dongmin Yu