Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using the CoraLite® Plus 647-conjugated version of this antibody, CL647-66467 (Heavy chain of Rabbit IgG antibody), at dilution of 1:200.
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using the CoraLite® Plus 647-conjugated version of this antibody, CL647-66467 (Heavy chain of Rabbit IgG antibody), at dilution of 1:200.
FC experiment of HeLa using CL647-66467
1X10^6 HeLa cells were intracellularly stained with Beta Catenin Polyclonal antibody (51067-2-AP) and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5) (red), or Control Antibody and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HeLa cells were intracellularly stained with Beta Catenin Polyclonal antibody (51067-2-AP) and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5) (red), or Control Antibody and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using the CoraLite® Plus 647-conjugated version of this antibody, CL647-66467 (Heavy chain of Rabbit IgG antibody), at dilution of 1:200.
FC (INTRA) Figures
FC experiment of HeLa using CL647-66467
1X10^6 HeLa cells were intracellularly stained with Beta Catenin Polyclonal antibody (51067-2-AP) and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5) (red), or Control Antibody and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Heavy chain of Rabbit IgG Monoclonal antibody
Catalog Number
CL647-66467
Citations
-
Dilutions
IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra)
Reactivity
rabbit
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
