ChromoTek GFP-Trap® Magnetic Particles M-270
GFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Magnetic Particles M-270.
Specificity
GFP, eGFP, CFP, YFP, BFP
Applications
IP, CoIP, ChIP, RIP
Conjugate
Magnetic Particles M-270
Cat no : gtd
Synonyms
Validation Data Gallery
Product Information
GFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Magnetic Particles M-270.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 1 pM |
Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP |
GeneID (NCBI) | 0 |
Coupled VHH | Monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment, recombinantly expressed |
Conjugate | Magnetic Particles M-270 |
Beads | ~ 2.8 µm (Magnetic, high throughput-compatible) |
Binding capacity | 10 µL slurry bind about 1 µg of recombinant GFP |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 1x PBS, Preservative: 0.09 % sodium azide |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS GFP-Trap Magnetic Particles M-270 |
Protocol |
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PROTOCOL GFP-Trap Magnetic articles M-270 |
White papers |
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Split Fluorescent Protein Technology (PDF) |
GFP-Trap? as affinity tag |
Unique Stability of the ChromoTek GFP-Trap:GFP Complex |
How to plan an Immunoprecipitation using the GFP-Trap |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) |
Brochure |
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GFP-Trap product brochure |
GFP-Trap Specificity |
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Fluorescent protein specificity table |
Publications
Application | Title |
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Nat Commun SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors. | |
Cell Rep WDR82/PNUTS-PP1 Prevents Transcription-Replication Conflicts by Promoting RNA Polymerase II Degradation on Chromatin. | |
Int J Mol Sci JAZF1, A Novel p400/TIP60/NuA4 Complex Member, Regulates H2A.Z Acetylation at Regulatory Regions. | |
mBio Functional Characterization of the m6A-Dependent Translational Modulator PfYTH.2 in the Human Malaria Parasite. | |
Cell Rep Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila. | |
BMC Mol Cell Biol KDM6A mutations promote acute cytoplasmic DNA release, DNA damage response and mitosis defects. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Anastasija (Verified Customer) (02-14-2022) | I used the GFP-trap dynabeads to perform co-IP of overexpressed GFP-tagged proteins in HEK293T cells. The pulldown was highly enriched in the bait and expected interactors, with low background. I am very satisfied with the performance of the product and will use it again.
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