ChromoTek GFP-Trap® Magnetic Particles M-270

GFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Magnetic Particles M-270.

Specificity

GFP, eGFP, CFP, YFP, BFP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Particles M-270

Publications

24

Reviews (1)

Cat no : gtd

Synonyms

GFP Trap Magnetic Particles M-270, GFP-Trap Magnetic Particles M-270, gtd, gtd-10, gtd-100, gtd-20, gtd-200, gtd-400, gfp, GFP-Trap® Magnetic Particles M-270, gtd-10, gtd-100, gtd-20, gtd-200, gtd-400

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Validation Data Gallery

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  Immunoprecipitation (IP) of GFP with GFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound.

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  Immunoprecipitation (IP) of GFP with GFP-Trap Magnetic Particles M-270: Coomassie and Western blot (I: Input, FT: Flow-Through, B: Bound).

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  Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.

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Product Information

GFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Magnetic Particles M-270.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 pM
• Specificity/Target: AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
• GeneID (NCBI): 0
• Coupled VHH: Monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment, recombinantly expressed
• Conjugate: Magnetic Particles M-270
• Beads: ~ 2.8 µm (Magnetic, high throughput-compatible)
• Binding capacity: 10 µL slurry bind about 1 µg of recombinant GFP
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 1x PBS, Preservative: 0.09 % sodium azide
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Publications

Application	Title
IP	Nat Commun; SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.; Authors - Seiichi Koike
IP	Cell Rep; WDR82/PNUTS-PP1 Prevents Transcription-Replication Conflicts by Promoting RNA Polymerase II Degradation on Chromatin.; Authors - Helga B Landsverk
IP	Int J Mol Sci; JAZF1, A Novel p400/TIP60/NuA4 Complex Member, Regulates H2A.Z Acetylation at Regulatory Regions.; Authors - Tara Procida
IP	mBio; Functional Characterization of the m6A-Dependent Translational Modulator PfYTH.2 in the Human Malaria Parasite.; Authors - Ameya Sinha
CoIP	Cell Rep; Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.; Authors - Nick Matinyan
CoIP	BMC Mol Cell Biol; KDM6A mutations promote acute cytoplasmic DNA release, DNA damage response and mitosis defects.; Authors - J Koch

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anastasija (Verified Customer) (02-14-2022)

I used the GFP-trap dynabeads to perform co-IP of overexpressed GFP-tagged proteins in HEK293T cells. The pulldown was highly enriched in the bait and expected interactors, with low background. I am very satisfied with the performance of the product and will use it again.

• Applications: Immunoprecipitation
• Cell Tissue Type: HEK293T