ChromoTek GFP-Trap® Magnetic Agarose, kit

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

Specificity

GFP and GFP derivates

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic agarose

Cat no : gtmak

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

DescriptionThe GFP-Trap Magnetic Agarose Kit contains GFP-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of GFP-fusion proteins and their interacting factors.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity15-20 μg of recombinant GFP per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 pM
Compatibility with mass spectrometryThe GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631358
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
GFP-Trap® Magnetic Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
IP

Nature

MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function.

Authors - Thomas Metzger
IP

Cell

Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex.

Authors - M Rhyan Puno
CoIP

Nat Med

Subcellular localization of coagulation factor II receptor-like 1 in neurons governs angiogenesis.

Authors - Jean-Sébastien Joyal
CoIP

Nat Cell Biol

The Machado-Joseph disease deubiquitylase ATX-3 couples longevity and proteostasis.

Authors - Kirsten Kuhlbrodt
IP

Mol Cell

Nucleo-cytosolic Shuttling of ARGONAUTE1 Prompts a Revised Model of the Plant MicroRNA Pathway.

Authors - Nicolas G Bologna
IP

Nat Chem Biol

Genetic code expansion in the mouse brain.

Authors - Russell J Ernst

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mona (Verified Customer) (09-02-2022)

I used the kit to co-immunoprecipitate a GFP tagged protein and it worked well for me. The efficiency was 100%. I would highly recommend it.

  • Applications: Immunoprecipitation