ChromoTek GFP-Trap® Agarose, kit

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads. The ChromoTek GFP-Trap® Agarose is a ready-to-use reagent.

Specificity

GFP and GFP derivates

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Cat no : gtak

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads. The ChromoTek GFP-Trap® Agarose is a ready-to-use reagent.

DescriptionThe GFP-Trap Agarose Kit contains GFP-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of GFP-fusion proteins and their interacting factors.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity25-30 µg of recombinant GFP per 25 µL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 pM
Compatibility with mass spectrometryThe GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631357
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
GFP-Trap® Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
IP

Nat Genet

ESR1 ligand-binding domain mutations in hormone-resistant breast cancer.

Authors - Weiyi Toy
IP

Nat Cell Biol

Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis.

Authors - Paulina Moreno-Layseca
IP

Nat Microbiol

CcrZ is a pneumococcal spatiotemporal cell cycle regulator that interacts with FtsZ and controls DNA replication by modulating the activity of DnaA.

Authors - Clement Gallay
IP

Sci Adv

A helical inner scaffold provides a structural basis for centriole cohesion.

Authors - Maeva Le Guennec
IP

Nat Commun

A signal motif retains Arabidopsis ER-α-mannosidase I in the cis-Golgi and prevents enhanced glycoprotein ERAD.

Authors - Jennifer Schoberer
CoIP

Nat Commun

SORLA regulates endosomal trafficking and oncogenic fitness of HER2.

Authors - Mika Pietilä