ChromoTek iST GFP-Trap® Kit for AP-MS sample preparation of GFP-fusion proteins

Kit for IP/ Co-IP of GFP-fusion proteins & sample preparation for mass spectrometry (MS)

Specificity

GFP and GFP derivates

Applications

IP, MS

Type

Nanobody

Conjugate

Agarose

Publications

3

Reviews (1)

Cat no : gtak-iST

Synonyms

GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  Schematic workflow of iST Nano-Trap® Kit

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  Save up to 40 hours on your mass spec experiments with the iST Nano-Trap® Kits

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Product Information

Kit for IP/ Co-IP of GFP-fusion proteins & sample preparation for mass spectrometry (MS)

• Description: The iST GFP-Trap® Kit enables researchers to process GFP-fusion proteins and their interacting partners for Mass Spectrometry analysis by including the ChromoTek GFP-Trap for immunoprecipitation/Co-IP of GFP-fusion proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls:

  • Reliable dentification of GFP-fusion proteins & interacting partners by MS

  • Identification of even low abundant/ low expressed GFP-fusion proteins

  • Superior processing of IP samples into clean peptide mixtures

  • Intuitive protocol for reproducible results

• Applications: IP, MS
• Specificity/Target: AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 25-30 µg of recombinant GFP per 25 µL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 1 pM
• Compatibility with mass spectrometry: The GFP-Trap® is optimized for on-bead digestion.
• Storage Buffer: 20% ethanol
• Storage Condition: Kit is shipped at ambient temperature. Upon receipt store GFP-Trap at 4°C and Digest at -20°C

Kit components

Component	Description
GFP-Trap Agarose	8 reactions plus 2 controls
Digest	Enzyme Trypsin-mix to digest proteins.
Resuspend buffer	Protease reconstitution buffer for enzymes.
Lyse buffer	Denature, reduce and alkylate proteins.
Stop solution	Stop the enzymatic activity.
Wash 1 buffer	Clean up peptides from hydrophobic contaminants.
Wash 2 buffer	Clean up peptides from hydrophilic contaminants.
Elution buffer	Elute the peptides from the cartridge.
LC-Load load	Load peptides on reversed-phase LC-MS column.
Cartridges	Cartridge for 1 to 100 µg protein starting material.
Waste tubes	Tube for collecting waste after washing steps.
Collection tubes	Tube for collecting peptides after elution.
Adapters	Enables placing a cartridge into a tube.
Caps	Cap to optionally close the cartridge’s bottom.

Publications

Application	Title
WB	J Biol Chem; Phagocytosis mediated by the human granulocyte receptor CEACAM3 is limited by the receptor-type protein tyrosine phosphatase PTPRJ.; Authors - Griseldis Goob
IP	PLoS One; Rab32 interacts with SNX6 and affects retromer-dependent Golgi trafficking.; Authors - Dieter Waschbüsch
IP	J Clin Invest; C16ORF70/MYTHO promotes healthy aging in C.elegans and prevents cellular senescence in mammals; Authors - Anais Franco-Romero

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Caitlin (Verified Customer) (06-23-2022)

It worked well!

• Applications: Immunoprecipitation
• Cell Tissue Type: Human IPSC astrocytes