ChromoTek GFP-Trap® Magnetic Particles M-270

GFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Magnetic Particles M-270.

Specificity

GFP and GFP derivates

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic Particles M-270

Cat no : gtd

Synonyms

GFP Trap Magnetic Particles M-270, GFP-Trap Magnetic Particles M-270, gtd, gtd-10, gtd-100, gtd-20, gtd-200, gtd-400, gfp, GFP-Trap® Magnetic Particles M-270, gtd-10, gtd-100, gtd-20, gtd-200, gtd-400



Product Information

GFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Magnetic Particles M-270.

DescriptionImmunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to magnetic particles. GFP-Trap Magnetic Particles M-270 is recommended when very large proteins/complexes are investigated and magnetic separation is needed for IP.

• IP and Co-IP of GFP-tagged proteins without size limitation

• Magnetic separation with easy and efficient washing of GFP-Trap Magnetic Particles M-270

• Automation and high throughput applications

• No heavy & light antibody chains, short incubation (5-30 min)

• Extraordinary binding, also under harsh conditions

• Very high affinity (KD=1 pM) to bind even low abundant proteins

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity2-2.8 μg of recombinant GFP per 25 μL bead slurry
ConjugateMagnetic Particles M-270, size: 2.8 µm
high throughput-compatible
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility10 mM DTT, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 0.2 % SDS, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 pM
Compatibility with mass spectrometryThe GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827592
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

Nat Cell Biol

ADAR1 downregulation by autophagy drives senescence independently of RNA editing by enhancing p16INK4a levels.

Authors - Xue Hao
IP

Neuron

Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.

Authors - Juliet Goldsmith
IP

Nat Commun

Spatial control of avidity regulates initiation and progression of selective autophagy.

Authors - David M Hollenstein
IP

Nat Commun

Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis.

Authors - Nicolas Butel
IP

EMBO J

Receptor-mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy.

Authors - Amelia E Ohnstad
ChIP

Plant Cell

Misregulation of MYB16 expression causes stomatal cluster formation by disrupting polarity during asymmetric cell divisions.

Authors - Shao-Li Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anastasija (Verified Customer) (02-14-2022)

I used the GFP-trap dynabeads to perform co-IP of overexpressed GFP-tagged proteins in HEK293T cells. The pulldown was highly enriched in the bait and expected interactors, with low background. I am very satisfied with the performance of the product and will use it again.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: HEK293T