Anticorps Polyclonal de lapin anti-VEGFC

VEGFC Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

34

Avis (2)

N° de cat : 22601-1-AP

Synonymes

Flt4 L, Flt4 ligand, VEGF C, VEGFC, VRP

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Galerie de données de validation

• 

  WB analysis using 22601-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22601-1-AP (VEGFC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22601-1-AP (VEGFC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 22601-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22601-1-AP (VEGFC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22601-1-AP (VEGFC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22601-1-AP (VEGFC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of RAW 264.7 using 22601-1-AP

  Immunofluorescent analysis of RAW 264.7 cells using 22601-1-AP (VEGFC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HCT 116, cellules RAW 264.7, cellules SW480
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 24 publications below
• IHC: See 13 publications below
• IF: See 5 publications below

Informations sur le produit

22601-1-AP cible VEGFC dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VEGFC Protéine recombinante Ag18182
• Nom complet: vascular endothelial growth factor C
• Masse moléculaire calculée: 419 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC035212
• Symbole du gène: VEGFC
• Identification du gène (NCBI): 7424
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VEGFC is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. The main function of VEGFC is to promote the growth of lymphatic vessels (lymphangiogenesis). It acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth, and migration.

Publications

Species	Application	Title
mouse	WB,IHC	J Extracell Vesicles; DUSP2 regulates extracellular vesicle-VEGF-C secretion and pancreatic cancer early dissemination.; Authors - Chu-An Wang
mouse	WB	Clin Transl Med; VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.; Authors - Qiu-Yue Lin
human	WB, IHC, IF	Cancer Lett; The Hippo-TAZ axis mediates vascular endothelial growth factor C in glioblastoma-derived exosomes to promote angiogenesis.; Authors - Zhengmin Wang
mouse	WB	Oxid Med Cell Longev; Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms.; Authors - Jie Bai
mouse	IHC	Front Pharmacol; Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway; Authors - Tong Zhou
human	WB	Aging (Albany NY); MicroRNA-330-3p promotes brain metastasis and epithelial-mesenchymal transition via GRIA3 in non-small cell lung cancer.; Authors - Chunhua Wei

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

No bands were observed. Does not represent the performance in other kind of samples

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

FH

hongxuan (Verified Customer) (11-12-2018)

we can detect the obvious signals in the embryo sections by using this primary antibody at a dilution of 1:50. Moreover, the background is very clear. Please forgive me that I cannot upload the files due to it's unpublished data.Thank you very much for you product.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: mouse embryo

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