VEGFD Polyclonal antibody

VEGFD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 26915-1-AP

Synonyms

c Fos induced growth factor, FIGF, VEGF D, VEGFD

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Validation Data Gallery

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  WB analysis using 26915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26915-1-AP (VEGFD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26915-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26915-1-AP (VEGFD antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26915-1-AP (VEGFD antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26915-1-AP (VEGFD antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, MCF-7 cells, rat heart tissue
• Positive IHC detected in: human lung cancer tissue, human heart tissue, human thyroid cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

The immunogen of 26915-1-AP is VEGFD Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VEGFD fusion protein Ag25552
• Full Name: c-fos induced growth factor (vascular endothelial growth factor D)
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC027948
• Gene Symbol: FIGF
• Gene ID (NCBI): 2277
• RRID: AB_2880683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VEGFD is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C.

Publications

Species	Application	Title
human	WB,IHC	Gastric Cancer; Cathepsin L promotes angiogenesis by regulating the CDP/Cux/VEGF-D pathway in human gastric cancer.; Authors - Tao Pan
mouse	IHC	Cereb Cortex; Prolonged Consumption of Sweetened Beverages Lastingly Deteriorates Cognitive Functions and Reward Processing in Mice.; Authors - Héloïse Hamelin
rat	IF	FASEB J; VEGF-D-mediated signaling in tendon cells is involved in degenerative processes.; Authors - Herbert Tempfer
mouse	IHC	Ann Transl Med; Clostridium butyricum alleviates dextran sulfate sodium-induced experimental colitis and promotes intestinal lymphatic vessel regeneration in mice.; Authors - Xing Chen
mouse	IHC	BMC Complement Med Ther; Effects of plant-based medicinal food on postoperative recurrence and lung metastasis of gastric cancer regulated by Wnt/β-catenin-EMT signaling pathway and VEGF-C/D-VEGFR-3 cascade in a mouse model; Authors - Lin Tian
mouse,human	WB,IF	Biochim Biophys Acta Mol Basis Dis; WTAP promotes macrophage recruitment and increases VEGF secretion via N6-methyladenosine modification in corneal neovascularization; Authors - Yanhui Bai