VEGFR2/KDR Polyclonal antibody

• Featured Product
• KD/KO Validated

VEGFR2/KDR Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

108

Reviews (3)

Cat no : 26415-1-AP

Synonyms

CD309, Fetal liver kinase 1, FLK 1, FLK1, KDR, Kinase insert domain receptor, VEGF Receptor 2, VEGFR, VEGFR 2, VEGFR2

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Validation Data Gallery

• 

  WB analysis using 26415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGFR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 26415-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGF Receptor 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 26415-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGF Receptor 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 26415-1-AP

  IP result of anti-VEGFR2/KDR (IP:26415-1-AP, 4ug; Detection:26415-1-AP 1:3000) with human placenta tissue lysate 600 ug.

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse heart tissue, mouse lung tissue, mouse placenta tissue, rat lung tissue
• Positive IP detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 79 publications below
• IHC: See 17 publications below
• IF: See 29 publications below
• IP: See 2 publications below
• FC: See 3 publications below
• CoIP: See 2 publications below

Product Information

26415-1-AP targets VEGFR2/KDR in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VEGFR2/KDR fusion protein Ag24589
• Full Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Calculated Molecular Weight: 1356 aa, 152 kDa
• Observed Molecular Weight: 150 kDa, 230 kDa
• GenBank Accession Number: BC131822
• Gene Symbol: KDR
• Gene ID (NCBI): 3791
• RRID: AB_2756527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Publications

Species	Application	Title
human	WB	Bioact Mater; A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds; Authors - Shunlai Shang
mouse	WB	J Exp Med; Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.; Authors - Michelle E LeBlanc
human	WB,IHC	Aging Dis; MSC-Derived Exosomes can Enhance the Angiogenesis of Human Brain MECs and Show Therapeutic Potential in a Mouse Model of Parkinson's Disease.; Authors - Chunling Xue
	WB,IF	J Neuroinflammation; Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization; Authors - Tianyi Shen
human	WB	Bioact Mater; Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid.; Authors - Wenjing Liu
human,mouse	WB,IHC	Biomed Pharmacother; Lychee seed polyphenol ameliorates DR via inhibiting inflammasome/apoptosis and angiogenesis in hRECs and db/db mice; Authors - Xiao-Hong Xiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Poulomi (Verified Customer) (07-02-2024)

The blot was good. It detected VEGFR2 in brain endothelial cells and Hek cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Endothelial Cells

FH

Sammy (Verified Customer) (01-20-2024)

This works well to detect VEGFR2 in HUVEC lysate. Ab diluted in 3% BSA PBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on angiogenesis study and use this for WB on HUVECs. It works very well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs

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