NME1 Polyclonal antibody

NME1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 11086-2-AP

Synonyms

NB, Granzyme A-activated DNase, Granzyme A activated DNase, GAAD, EC:2.7.4.6

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Validation Data Gallery

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  WB analysis of HeLa using 11086-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells using 11086-2-AP

  WB result of 10086-2-AP (NME1 antibody).

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  WB analysis of A549 using 11086-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11086-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11086-2-AP

  IP result of anti-NME1 (IP:11086-2-AP, 4ug; Detection:11086-2-AP 1:500) with A549 cells lysate 2800ug.

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  IHC staining of human liver using 11086-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11086-2-AP (NME1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11086-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11086-2-AP (NME1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11086-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 11086-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas cancer using 11086-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of MCF-7 using 11086-2-AP

  Immunofluorescent analysis of MCF-7 cells using 11086-2-AP (NME1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, multi-cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human lung cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

11086-2-AP targets NME1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NME1 fusion protein Ag1548
• Full Name: non-metastatic cells 1, protein (NM23A) expressed in
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC018994
• Gene Symbol: NME1
• Gene ID (NCBI): 4830
• RRID: AB_2152689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NME1 is also named as NDPKA(Nucleoside diphosphate kinase A), NM23(Metastasis inhibition factor nm23),GAAD(Granzyme A-activated DNase) and belongs to the NDK family.It is a major role in the synthesis of nucleoside triphosphates other than ATP and required for neural development including neural patterning and cell fate determination.It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
human	WB	Redox Biol; Regulation of metastasis suppressor NME1 by a key metabolic cofactor coenzyme A.; Authors - Bess Yi Kun Yu
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki
human	IF	J Transl Med; Identification and validation of smoking-related genes in lung adenocarcinoma using an in vitro carcinogenesis model and bioinformatics analysis.; Authors - Jin Wang
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang
human	IF	Oncol Rep; LINC01816 promotes the migration, invasion and epithelial‑mesenchymal transition of thyroid carcinoma cells by sponging miR‑34c‑5p and regulating CRABP2 expression levels.; Authors - Hongyuan Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chantal (Verified Customer) (10-04-2022)

Specific band appeared after 1 minute of exposure. This antibody works on mouse and human cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MEFs, Hela, HEK293FT

NME1 Antibody Western Blot validation (1:1000 dilution) in MEFs, Hela, HEK293FT (Cat no:11086-2-AP)