Anticorps Polyclonal de lapin anti-VDAC3

VDAC3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 14451-1-AP

Synonymes

HD VDAC3, hVDAC3, VDAC 3, VDAC3

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Galerie de données de validation

• 

  WB analysis of mouse heart using 14451-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14451-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14451-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14451-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14451-1-AP (VDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14451-1-AP (VDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14451-1-AP (VDAC3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human osteosarcoma using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14451-1-AP (VDAC3 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu d'ostéosarcome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 2 publications below

Informations sur le produit

14451-1-AP cible VDAC3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VDAC3 Protéine recombinante Ag5960
• Nom complet: voltage-dependent anion channel 3
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC056870
• Symbole du gène: VDAC3
• Identification du gène (NCBI): 7419
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.

Publications

Species	Application	Title
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
mouse	WB	Mol Ther Nucleic Acids; Salvia miltiorrhiza-Derived Sal-miR-58 Induces Autophagy and Attenuates Inflammation in Vascular Smooth Muscle Cells.; Authors - Yan Qin
human	IHC	Oncotarget; Proteomics-based identification of VDAC1 as a tumor promoter in cervical carcinoma.; Authors - Changlin Zhang
	WB	J Cell Sci; Doublet 7 shortening, doublet 5-preferential poly-Glu reduction, and beating stall of sperm flagella in Ttll9-/- mice.; Authors - Alu Konno
rat	WB	Int J Mol Med; Herceptin induces ferroptosis and mitochondrial dysfunction in H9c2 cells.; Authors - Lei Sun
human	WB	Front Aging Neurosci; The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis.; Authors - Yue-Qi Wang

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