VDAC2 Polyclonal antibody

VDAC2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11663-1-AP

Synonyms

hVDAC2, VDAC 2, VDAC2



Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman prostate cancer tissue, human prostate tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11663-1-AP targets VDAC2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VDAC2 fusion protein Ag2266
Full Name voltage-dependent anion channel 2
Calculated molecular weight 294 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000165
Gene symbol VDAC2
Gene ID (NCBI) 7417
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.

Protocols

Product Specific Protocols
WB protocol for VDAC2 antibody 11663-1-APDownload protocol
IHC protocol for VDAC2 antibody 11663-1-APDownload protocol
IP protocol for VDAC2 antibody 11663-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis.

Authors - Shintaro Mise
mouseIP

Autophagy

MYBL2 guides autophagy suppressor VDAC2 in the developing ovary to inhibit autophagy through a complex of VDAC2-BECN1-BCL2L1 in mammals.

Authors - Jia Yuan
human,mouseWB,IF

Cell Death Differ

SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy.

Authors - Ying Zhang
mouseWB

Proc Natl Acad Sci U S A

SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.

Authors - Haruhiko Miyata
humanWB

EMBO Mol Med

Conformational change of adenine nucleotide translocase-1 mediates cisplatin resistance induced by EBV-LMP1.

Authors - Lin Zhao
humanIHC

Oncotarget

Proteomics-based identification of VDAC1 as a tumor promoter in cervical carcinoma.

Authors - Changlin Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jun (Verified Customer) (06-12-2022)

Works very well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
VDAC2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11663-1-AP)