Anticorps Polyclonal de lapin anti-Stathmin 1

• Phare
• Validé par KD/KO

Stathmin 1 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, FC (Intra), ELISA, IF

Conjugaison

Non conjugué

Publications

24

Avis (1)

N° de cat : 11157-1-AP

Synonymes

STMN1, stathmin 1/oncoprotein 18, Stathmin, OP18, Leukemia-associated phosphoprotein p18

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Galerie de données de validation

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  WB analysis of Jurkat using 11157-1-AP

  WB results of Stathmin antibody (11157-1-AP, 1:20000) with si-control and si-Stathmin transfected Jurkat cells..

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  WB analysis using 11157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11157-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11157-1-AP (Stathmin 1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 11157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HeLa using 11157-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Stathmin 1 (11157-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules PC-12
• Résultats positifs en IHC: tissu de gliome humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 19 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Informations sur le produit

11157-1-AP cible Stathmin 1 dans les applications de WB, IHC, FC (Intra), ELISA, IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Stathmin 1 Protéine recombinante Ag1633
• Nom complet: stathmin 1/oncoprotein 18
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC014353
• Symbole du gène: Stathmin 1
• Identification du gène (NCBI): 3925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Stem Cell; Continuous clonal labeling reveals uniform progenitor potential in the adult exocrine pancreas.; Authors - Sophie C Lodestijn
rat	WB	Chemosphere; Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.; Authors - Mingdan You
human	WB	Int J Nanomedicine; Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.; Authors - Chen Li
human	WB	Oncogene; KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.; Authors - Lei Zhao ; KD Validated
human	IHC	Oncotarget; Stathmin and phospho-stathmin protein signature is associated with survival outcomes of breast cancer patients.; Authors - Xia-Ying Kuang
human	IHC	Neuroimage; Developmental dynamics of the periventricular parietal crossroads of growing cortical pathways in the fetal brain - In vivo fetal MRI with histological correlation.; Authors - Ruxandra-Iulia Milos

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Richard (Verified Customer) (10-08-2019)

Antibody works well at 1:4000 and gives a single band, have no issues with it.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: HeLa

Stathmin 1 Antibody Western Blot, validation (1:4000 dilution) in HeLa (Cat no:11157-1-AP)

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