STMN3 Polyclonal antibody

• Featured Product
• KD/KO Validated

STMN3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11311-1-AP

Synonyms

SCG10 like protein, SCLIP, Stathmin 3, stathmin like 3, STMN3

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Validation Data Gallery

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  WB analysis using 11311-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11311-1-AP (STMN3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11311-1-AP targets STMN3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STMN3 fusion protein Ag1853
• Full Name: stathmin-like 3
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: BC009381
• Gene Symbol: STMN3
• Gene ID (NCBI): 50861
• RRID: AB_2197399
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stathmin family phosphoproteins participate in the control of microtubule dynamics and have been proposed to be involved in the control of neuronal differentiation. Neuron specific Stathmin-3 (STMN3) is a novel STAT3 (signal transducer and activator of transcription 3) interacting protein. STAT3 is a key contributor to cancer cell migration and invasion. STMN3 exhibits microtubule-destabilizing activity, which is antagonized by STAT3. SCG10 is a widely studied and recognized neuronal differentiation marker, STMN3 is a SCG10-like protein (SCLIP) and is present from the earliest stages of hippocampal neuron differentiation in culture at vesicle-like structures following dynamic microtubules. RNAi mediated inhibition of this gene resulted in increased axonal branching.

Publications

Species	Application	Title
human	WB, IHC	Cancer Res; Coordinated expression of stathmin family members by far upstream sequence element-binding protein-1 increases motility in non-small cell lung cancer.; Authors - Stephan Singer
human	WB	Cancer Res; RUNX1 is a driver of renal cell carcinoma correlating with clinical outcome.; Authors - Nicholas Rooney
mouse	WB,IF	Elife; HAT cofactor TRRAP modulates microtubule dynamics via SP1 signaling to prevent neurodegeneration.; Authors - Alicia Tapias
human	WB	Mol Cancer Ther; PMID: 23543364; Authors - Xie Xuemei X ; KD Validated
mouse	WB	J Biol Chem; The Stathmin-2 membrane targeting domain is required for axon protection and regulated degradation by DLK signaling; Authors - Emma J C Thornburg-Suresh
	WB	BMC Cell Biol; CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited.; Authors - Matthew A Helsby