SURVIVIN Polyclonal antibody

• Featured Product
• KD/KO Validated

SURVIVIN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

150

Reviews (2)

Cat no : 10508-1-AP

Synonyms

BIRC5, IAP4, EPR 1, BIRC 5, Baculoviral IAP repeat-containing protein 5

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Validation Data Gallery

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  WB analysis of Jurkat using 10508-1-AP

  WB result of Survivin antibody (10508-1-AP, 1:5000) with si-control and si-Survivin transfected Jurkat cells..

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  WB analysis using 10508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10508-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10508-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of U2OS using 10508-1-AP

  IP result of anti-SURVIVIN (IP:10508-1-AP, 4ug; Detection:10508-1-AP 1:1000) with U2OS cells lysate 440 ug.

• 

  IP experiment of Jurkat using 10508-1-AP

  IP result of anti-SURVIVIN (IP:10508-1-AP, 4ug; Detection:10508-1-AP 1:300) with Jurkat cells lysate 2120ug.

• 

  IHC staining of human colon cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10508-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SURVIVIN antibody (10508-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 10508-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug SURVIVIN Polyclonal antibody (10508-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, Jurkat cells, NIH/3T3 cells, A549 cells, MCF-7 cells, A431 cells, Ramos cells
• Positive IP detected in: U2OS cells, Jurkat cells
• Positive IHC detected in: human colon cancer tissue, human urothelial carcinoma tissue, human stomach cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 135 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Product Information

10508-1-AP targets SURVIVIN in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, canine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SURVIVIN fusion protein Ag0788
• Full Name: baculoviral IAP repeat-containing 5
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: BC008718
• Gene Symbol: Survivin
• Gene ID (NCBI): 332
• RRID: AB_2064048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

Publications

Species	Application	Title
human	WB	Biomaterials; PMID: 29028548; Authors - Yudong Song
human,mouse	WB	Theranostics; Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI; Authors - Bojun Li
human	WB	Autophagy; Elaiophylin, a novel autophagy inhibitor, exerts antitumor activity as a single agent in ovarian cancer cells.; Authors - Xuejiao Zhao
human	WB	Circ Res; Iron Homeostasis and Pulmonary Hypertension: Iron Deficiency Leads to Pulmonary Vascular Remodeling in the Rat.; Authors - Emanuele Cotroneo
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	WB	Cancer Lett; SKP2 promotes the metastasis of pancreatic ductal adenocarcinoma by suppressing TRIM21-mediated PSPC1 degradation; Authors - Jiahui Yuan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Susmita (Verified Customer) (08-21-2020)

Giving good result and crisp band in Western Blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Pancreatic cell line

SURVIVIN Antibody Western Blot validation (1:1000 dilution) in Pancreatic cell line (Cat no:10508-1-AP)

FH

Iram (Verified Customer) (08-14-2020)

We got very sharp and clean band of survivin with this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human colon cancer

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