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STAT3 Polyclonal antibody
STAT3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10253-2-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, rat brain tissue, mouse lung tissue, Jurkat cells, A431 cells, mouse brain tissue, K-562 cells, mouse heart tissue, MCF-7 cells, NIH/3T3 cells, HeLa cells, PC-12 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human stomach cancer tissue, human colon cancer tissue, human cervical cancer tissue, mouse colon tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10253-2-AP targets STAT3 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish, marmoset|
|Host / Isotype||Rabbit / IgG|
|Immunogen||STAT3 fusion protein Ag0360|
|Full Name||signal transducer and activator of transcription 3 (acute-phase response factor)|
|Calculated molecular weight||770 aa, 88 kDa|
|Observed molecular weight||88 kDa|
|GenBank accession number||BC000627|
|Gene ID (NCBI)||6774|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.
|Product Specific Protocols|
|WB protocol for STAT3 antibody 10253-2-AP||Download protocol|
|IHC protocol for STAT3 antibody 10253-2-AP||Download protocol|
|IF protocol for STAT3 antibody 10253-2-AP||Download protocol|
|IP protocol for STAT3 antibody 10253-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Daniele (Verified Customer) (04-29-2022)
It works very well for WB, IF and IP
Yuan (Verified Customer) (01-23-2020)
Very good Stat3 antibody for western blot. Strong signal at 1:5000 dilution. Highly recommend.
Daniele (Verified Customer) (11-25-2019)
It works very well both in western blot and in immunoprecipitation.