STAT3 Polyclonal antibody

• Featured Product
• KD/KO Validated

STAT3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

477

Reviews (3)

Cat no : 10253-2-AP

Synonyms

STAT 3, HIES, APRF, Acute-phase response factor

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Validation Data Gallery

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  WB analysis using 10253-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10253-2-AP

  WB result of STAT3 antibody (10253-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT3 transfected A549 cells.

• 

  WB analysis using 10253-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10253-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10253-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10253-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10253-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10253-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10253-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10253-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10253-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10253-2-AP

  IP result of anti-STAT3 (IP:10253-2-AP, 3ug; Detection:10253-2-AP 1:2000) with HeLa cells lysate 3000ug.

• 

  IHC staining of human stomach cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10253-2-AP (STAT3 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 10253-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10253-2-AP (STAT3 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 10253-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI (blue).

• 

  IF Staining of HeLa using 10253-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed IFN alpha treated HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 10253-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 10253-2-AP

  Immunofluorescent analysis of HeLa cells using 10253-2-AP (STAT3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of Ramos using 10253-2-AP

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human STAT3 (10253-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-12 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue, human colon cancer tissue, human cervical cancer tissue, mouse colon tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, IFN alpha treated HeLa cells
• Positive FC (Intra) detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 24 publications below
• WB: See 441 publications below
• IHC: See 34 publications below
• IF: See 33 publications below
• IP: See 8 publications below
• CoIP: See 8 publications below
• ChIP: See 13 publications below
• RIP: See 1 publications below

Product Information

10253-2-AP targets STAT3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish, bovine, marmoset
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAT3 fusion protein Ag0360
• Full Name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Calculated Molecular Weight: 770 aa, 88 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC000627
• Gene Symbol: STAT3
• Gene ID (NCBI): 6774
• RRID: AB_2302876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human,mouse	WB	Cell Metab; Lactate modulates iron metabolism by binding soluble adenylyl cyclase; Authors - Wei Liu
mouse	WB	Brain Behav Immun; l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.; Authors - Danqing Xin
mouse	WB	ACS Appl Mater Interfaces; A Versatile Nanoplatform for Broad-Spectrum Immunotherapy by Reversing the Tumor Microenvironment; Authors - Ao Chen
mouse	WB	J Nanobiotechnology; Nanocomposites based on nanoceria regulate the immune microenvironment for the treatment of polycystic ovary syndrome; Authors - Sisi Yan
rat	WB	Adv Healthc Mater; Dual-Barb Microneedle with JAK/STAT Inhibitor-Loaded Nanovesicles Encapsulation for Tendinopathy; Authors - Minhao Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniele (Verified Customer) (04-29-2022)

It works very well for WB, IF and IP

• Applications: Western Blot, Immunofluorescence, Other
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: TNBC

FH

Yuan (Verified Customer) (01-23-2020)

Very good Stat3 antibody for western blot. Strong signal at 1:5000 dilution. Highly recommend.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Hela

FH

Daniele (Verified Customer) (11-25-2019)

It works very well both in western blot and in immunoprecipitation.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000 (western blot)
• Cell Tissue Type: MDA MB 468 and A375

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