ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

404

Reviews (2)

Cat no : 16443-1-AP

Synonyms

ERK, ERK 2, ERK1/2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, MAPK2, p40, p41, p41mapk, p42 MAPK, P42MAPK, PRKM1, PRKM2

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Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 16443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16443-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16443-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16443-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16443-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 16443-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16443-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16443-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16443-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 16443-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 16443-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 16443-1-AP (ERK1/2 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 16443-1-AP

  Immunofluorescent analysis of HeLa cells using 16443-1-AP (ERK1/2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, A431 cells, mouse kidney tissue, HEK-293 cells, HeLa cells, PC-3 cells, rat kidney tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 394 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

16443-1-AP targets ERK1/2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, sheep, eriocheir sinensis
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERK1/2 fusion protein Ag9772
• Full Name: mitogen-activated protein kinase 1
• Calculated Molecular Weight: 360 aa, 41 kDa
• Observed Molecular Weight: 38-44 kDa
• GenBank Accession Number: BC017832
• Gene Symbol: ERK2
• Gene ID (NCBI): 5594
• RRID: AB_10603369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publications

Species	Application	Title
human	WB	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
human	WB	Cancer Lett; Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma; Authors - Qianyu Shi
rat	WB	Adv Sci (Weinh); An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress.; Authors - Jing Liu
human	WB	J Hazard Mater; ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.; Authors - Xuan Lai
human	WB,IP	Cell Death Dis; 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer; Authors - Yanan Wang
human	WB	Mol Ther; Exosomal miR-1246 from glioma patient body fluids drives the differentiation and activation of myeloid-derived suppressor cells.; Authors - Wei Qiu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

good results on human lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Tom (Verified Customer) (09-29-2020)

10ug of HEK293T total cell lysate in SDS loading buffer was loaded and ran on a 4-20% gradient gel. Blocking was performed in 5% BSA in TBS for 1 hour then membrane incubated at 4 degrees overnight with anti-ERK 1/2 antibody (1:1000) in block. A goat anti-rabbit HRP secondary antibody (1:10,000) was used.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

ERK1/2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:16443-1-AP)

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