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Annexin A2 Polyclonal antibody
Annexin A2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 11256-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, mouse placenta tissue, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, HepG2 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver tissue, human breast cancer tissue, human hepatocirrhosis tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | T-47D cells, MCF-7 cells, HeLa cells |
Positive FC (Intra) detected in | K-562 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 10 publications below |
WB | See 51 publications below |
IHC | See 14 publications below |
IF | See 14 publications below |
IP | See 8 publications below |
RIP | See 2 publications below |
Product Information
11256-1-AP targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, monkey, chicken, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Annexin A2 fusion protein Ag1777 |
Full Name | annexin A2 |
Calculated Molecular Weight | 339 aa, 39 kDa |
Observed Molecular Weight | 35-40 kDa |
GenBank Accession Number | BC016774 |
Gene Symbol | Annexin A2 |
Gene ID (NCBI) | 302 |
RRID | AB_2057311 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
IHC protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
IF protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
IP protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death. | ||
Nat Chem Biol YAP-dependent proliferation by a small molecule targeting annexin A2.
| ||
Nucleic Acids Res Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing. | ||
EBioMedicine Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Ioannis (Verified Customer) (09-13-2023) | Works perfectly for Zebrafish (IHC).
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