Annexin A2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Annexin A2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Conjugate

Unconjugated

Publications

66

Reviews (1)

Cat no : 11256-1-AP

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II

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Validation Data Gallery

• 

  WB analysis using 11256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11256-1-AP

  WB result of Annexin A2 (11256-1-AP, 1:1000) with si-Control and si-Annexin A2 transfected HEK293 cells.

• 

  WB analysis of rat uterus using 11256-1-AP

  rat uterus tissue were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 11256-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11256-1-AP (Annexin A2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11256-1-AP

  IP result of anti-Annexin A2 (IP:11256-1-AP, 4ug; Detection:11256-1-AP 1:3000) with HeLa cells lysate 2640 ug.

• 

  IHC staining of human liver using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11256-1-AP (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse kidney using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11256-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11256-1-AP (Annexin A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of T-47D using 11256-1-AP

  Immunofluorescent analysis of (4% PFA) fixed T-47D cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 11256-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of MCF-7 using 11256-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11256-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11256-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A2 antibody (11256-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 11256-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11256-1-AP (Annexin A2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of K-562 using 11256-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Annexin A2 (11256-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse placenta tissue, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue, human bowen disease, human breast cancer tissue, human hepatocirrhosis tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, mouse kidney tissue, mouse liver tissue, mouse placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: T-47D cells, MCF-7 cells, HeLa cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 51 publications below
• IHC: See 14 publications below
• IF: See 14 publications below
• IP: See 9 publications below
• ELISA: See 1 publications below
• RIP: See 2 publications below

Product Information

11256-1-AP targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Annexin A2 fusion protein Ag1777
• Full Name: annexin A2
• Calculated Molecular Weight: 339 aa, 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC016774
• Gene Symbol: Annexin A2
• Gene ID (NCBI): 302
• RRID: AB_2057311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Publications

Species	Application	Title
human		Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
human	WB	Nat Chem Biol; YAP-dependent proliferation by a small molecule targeting annexin A2.; Authors - Sophia Z Shalhout ; KD Validated
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
mouse	WB	Nucleic Acids Res; Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.; Authors - Ken Asada
human	IF,IHC	EBioMedicine; Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder.; Authors - Xiao Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ioannis (Verified Customer) (09-13-2023)

Works perfectly for Zebrafish (IHC).

• Applications: Immunohistochemistry

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