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Annexin A2 Polyclonal antibody
Annexin A2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 11256-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, mouse placenta tissue, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, HepG2 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver tissue, human bowen disease, human breast cancer tissue, human hepatocirrhosis tissue, human lung cancer tissue, human oesophagus cancer tissue, human prostate cancer tissue, mouse kidney tissue, mouse liver tissue, mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | T-47D cells, MCF-7 cells, HeLa cells |
Positive FC (Intra) detected in | K-562 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 10 publications below |
WB | See 52 publications below |
IHC | See 14 publications below |
IF | See 16 publications below |
IP | See 9 publications below |
ELISA | See 1 publications below |
RIP | See 2 publications below |
Product Information
11256-1-AP targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, monkey, chicken, zebrafish, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Annexin A2 fusion protein Ag1777 |
Full Name | annexin A2 |
Calculated Molecular Weight | 339 aa, 39 kDa |
Observed Molecular Weight | 35-40 kDa |
GenBank Accession Number | BC016774 |
Gene Symbol | Annexin A2 |
Gene ID (NCBI) | 302 |
RRID | AB_2057311 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
IHC protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
IF protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
IP protocol for Annexin A2 antibody 11256-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death. | ||
Nat Chem Biol YAP-dependent proliferation by a small molecule targeting annexin A2.
| ||
Nucleic Acids Res Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing. | ||
EBioMedicine Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Ioannis (Verified Customer) (09-13-2023) | Works perfectly for Zebrafish (IHC).
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