Anticorps Polyclonal de lapin anti-RB1

• Phare
• Validé par KD/KO

RB1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

54

Avis (2)

N° de cat : 10048-2-Ig

Synonymes

OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1

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Galerie de données de validation

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  WB analysis using 10048-2-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 10048-2-Ig (RB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10048-2-Ig

  Non-treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 30376-1-AP (Phospho-RB1 (Ser807/811) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RB1 antibody (10048-2-Ig) subsequently.

• 

  IP experiment of A431 using 10048-2-Ig

  IP result of anti-RB1 (IP:10048-2-Ig, 3ug; Detection:10048-2-Ig 1:500) with A431 cells lysate 3000 ug.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules Jurkat traitées à la calyculine A, cellules MCF-7
• Résultats positifs en IP: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 45 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

10048-2-Ig cible RB1 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, poisson-zèbre
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RB1 Protéine recombinante Ag0006
• Nom complet: retinoblastoma 1
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC040540
• Symbole du gène: RB1
• Identification du gène (NCBI): 5925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against human RB1 fusion protein.

Publications

Species	Application	Title
	IHC	Nat Genet; Zbtb7a suppresses prostate cancer through repression of a Sox9-dependent pathway for cellular senescence bypass and tumor invasion.; Authors - Wang Guocan G
human	IHC	Gastroenterology; Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.; Authors - Yuhong Luo
human	WB, IHC	J Clin Invest; TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.; Authors - Zheng Hu
human	WB	Nat Commun; Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.; Authors - Kai Yuan
human	WB	Theranostics; Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.; Authors - Shuai Huang
human	WB	Clin Cancer Res; Zinc finger nucleases targeting the human papillomavirus E7 oncogene induce E7 disruption and a transformed phenotype in HPV 16/18-positive cervical cancer cells.; Authors - Wencheng Ding

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Georgina (Verified Customer) (07-04-2022)

This antibody gave some background staining, however the band of interest was clear.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 5000
• Cell Tissue Type: Human HepG2 cells

FH

Kyle (Verified Customer) (12-04-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: small cell lung cancer

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