p107 Polyclonal antibody

• Featured Product
• KD/KO Validated

p107 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 13354-1-AP

Synonyms

CP107, p107, PRB1, RBL1, retinoblastoma like 1 (p107), Retinoblastoma like protein 1

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Validation Data Gallery

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  WB analysis of mouse testis using 13354-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13354-1-AP (p107 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 13354-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13354-1-AP (p107 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13354-1-AP

  IP result of anti-p107 (IP:13354-1-AP, 4ug; Detection:13354-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse testis tissue, COLO 320 cells
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IP: See 1 publications below

Product Information

13354-1-AP targets p107 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, non-human primate
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p107 fusion protein Ag4146
• Full Name: retinoblastoma-like 1 (p107)
• Calculated Molecular Weight: 1068 aa, 119 kDa
• Observed Molecular Weight: 119 kDa
• GenBank Accession Number: BC032247
• Gene Symbol: p107
• Gene ID (NCBI): 5933
• RRID: AB_2238024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Retinoblastoma-like protein 1, also named RBL1, p107, is a member of E1A-binding proteins, which contains a A/B pocket, or pRb. Via this structure, RBL1 can bind to viral oncoproteins and other perteins containing LXCXE motif. It regulates cell proliferation via phosphorylation-sensitive interactions with E2F transcription factors and other proteins. Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression.(uniprot). It has been reported there are two transcirpts variants of RBL1, which encode a 119-kd protein and a 68-kd protein(PMID:7490090).

Publications

Species	Application	Title
mouse	WB,IP	Nat Commun; p107 mediated mitochondrial function controls muscle stem cell proliferative fates.; Authors - Debasmita Bhattacharya
human	WB	Cancers (Basel); RBL1/p107 Expression Levels Are Modulated by Multiple Signaling Pathways.; Authors - Elisa Ventura
human	WB	Front Cell Dev Biol; miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis; Authors - Dong Pan
human	WB	Oncotarget; Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells.; Authors - Dong Pan ; KD Validated
mouse	WB	Biochem Pharmacol; Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms.; Authors - Ji Young Kim
human	WB	Mol Pharm; Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.; Authors - Xiaoman Chen