E2F1 Polyclonal antibody

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  WB analysis of HeLa using 12171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12171-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12171-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12171-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12171-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12171-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12171-1-AP (E2F1 antibody) at dilution of 1:50 (under 10x lens).

• Featured Product
• KD/KO Validated

E2F1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12171-1-AP

Publications(54)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Retinoblastoma-associated protein 1, RBBP-3, RBAP-1, pRB-binding protein E2F-1, E2F-1

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, HeLa cells, mouse brain tissue, NIH/3T3 cells, HepG2 cells, Jurkat cells, LNCaP cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 48 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12171-1-AP targets E2F1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, artemia sinica
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2813

  Product name: Recombinant human E2F1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 88-438 aa of BC050369

  Sequence: VKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF

• Full Name: E2F transcription factor 1
• Calculated Molecular Weight: 437 aa, 47 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC050369
• Gene Symbol: E2F1
• Gene ID (NCBI): 1869
• RRID: AB_2096958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01094
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of E2F1 protein, specifically recognizes the 47kd human E2F1 protein. The sumoylated E2F1 is bout 55 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	J Clin Invest; TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.; Authors - Zheng Hu
human	WB	Nat Commun; Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.; Authors - Dan Su
human	WB	Cancer Lett; The oncoprotein HBXIP up-regulates SCG3 through modulating E2F1 and miR-509-3p in hepatoma cells.; Authors - Yue Wang ; KD Validated
mouse	IHC	Int J Nanomedicine; E7-Targeted Nanotherapeutics for Key HPV Afflicted Cervical Lesions by Employing CRISPR/Cas9 and Poly (Beta-Amino Ester); Authors - Jinfeng Xiong
human	IF	Mol Ther; Nanoparticles Based on Poly (β-Amino Ester) and HPV16-Targeting CRISPR/shRNA as Potential Drugs for HPV16-Related Cervical Malignancy.; Authors - Da Zhu
human	WB	Cell Death Dis; SEC61G promotes breast cancer development and metastasis via modulating glycolysis and is transcriptionally regulated by E2F1.; Authors - Jingjing Ma ; KD Validated