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  • KD/KO Validated

CDK4 Polyclonal antibody

CDK4 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, rat, mouse and More (4)


WB, IP, IF, FC, RIP, IHC, CoIP, ELISA, Cell treatment



Cat no : 11026-1-AP


CDK4, Cell division protein kinase 4, CMM3, cyclin dependent kinase 4, PSK J3

Tested Applications

Positive WB detected inHeLa cells, rat pancreas tissue, HepG2 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11026-1-AP targets CDK4 in WB, IP, IF, FC, RIP, IHC, CoIP, ELISA, Cell treatment applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, sheep, zebrafish, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK4 fusion protein Ag1505
Full Name cyclin-dependent kinase 4
Calculated Molecular Weight 34 kDa
Observed Molecular Weight30-34 kDa
GenBank Accession NumberBC010153
Gene Symbol CDK4
Gene ID (NCBI) 1019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).


Product Specific Protocols
WB protocol for CDK4 antibody 11026-1-APDownload protocol
IHC protocol for CDK4 antibody 11026-1-APDownload protocol
IF protocol for CDK4 antibody 11026-1-APDownload protocol
IP protocol for CDK4 antibody 11026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.

Authors - Esteban A Orellana

Mol Cancer

The circACTN4 interacts with FUBP1 to promote tumorigenesis and progression of breast cancer by regulating the expression of proto-oncogene MYC.

Authors - Xiaosong Wang

Nat Commun

CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.

Authors - Youzhou Sang

Nat Commun

Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.

Authors - Dan Su

J Pineal Res

Mitochondrial cytochrome P450 (CYP) 1B1 is responsible for melatonin-induced apoptosis in neural cancer cells.

Authors - Zhenlong Yu

Cancer Res

CDK4/6 inhibition enhances oncolytic virus efficacy by potentiating tumor-selective cell killing and T cell activation in refractory glioblastoma.

Authors - Deyin Guo
  • KD Validated