CDK4 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDK4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

395

Cat no : 11026-1-AP

Synonyms

PSK J3, EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3

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Validation Data Gallery

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  WB analysis using 11026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11026-1-AP (CDK4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11026-1-AP

  WB result of CDK4 antibody (11026-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK4 transfected HeLa cells.

• 

  WB analysis of rat pancreas using 11026-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11026-1-AP (CDK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11026-1-AP (CDK4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11026-1-AP

  IP result of anti-CDK4 (IP:11026-1-AP, 3ug; Detection:11026-1-AP 1:1000) with HeLa cells lysate 4000ug.

• 

  IHC staining of human lung cancer using 11026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11026-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11026-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11026-1-AP (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 11026-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CDK4 antibody (11026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 11026-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CDK4 Polyclonal antibody (11026-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, rat pancreas tissue, HepG2 cells, MCF-7 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 383 publications below
• IHC: See 29 publications below
• IF: See 6 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below
• RIP: See 1 publications below

Product Information

11026-1-AP targets CDK4 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, zebrafish, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK4 fusion protein Ag1505
• Full Name: cyclin-dependent kinase 4
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC010153
• Gene Symbol: CDK4
• Gene ID (NCBI): 1019
• RRID: AB_2078702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).

Publications

Species	Application	Title
mouse	WB	Mol Cell; METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.; Authors - Esteban A Orellana
human	IHC,WB	Mol Cancer; The circACTN4 interacts with FUBP1 to promote tumorigenesis and progression of breast cancer by regulating the expression of proto-oncogene MYC.; Authors - Xiaosong Wang
human	WB	Sci Adv; The p23 co-chaperone is a succinate-activated COX-2 transcription factor in lung adenocarcinoma tumorigenesis; Authors - Zhenlong Yu
human	WB	Nat Commun; CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.; Authors - Youzhou Sang
human	WB	Nat Commun; Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.; Authors - Dan Su
human	WB	J Pineal Res; Mitochondrial cytochrome P450 (CYP) 1B1 is responsible for melatonin-induced apoptosis in neural cancer cells.; Authors - Zhenlong Yu

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