Anticorps Polyclonal de lapin anti-Osteocalcin

Osteocalcin Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

178

Avis (4)

N° de cat : 23418-1-AP

Synonymes

BGLAP, Gamma-carboxyglutamic acid-containing protein, Bone Gla protein, BGP

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Galerie de données de validation

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  IHC staining of mouse testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23418-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IHC staining of rat testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human osteosarcoma using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 23418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23418-1-AP (Osteocalcin Antibody) at dilution of 1:50.

• 

  IF Staining of U2OS using 23418-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Osteocalcin antibody (23418-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 23418-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using Osteocalcin antibody (23418-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 23418-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human Osteocalcin (23418-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IHC: tissu testiculaire de souris, tissu d'ostéosarcome humain, tissu rénal de souris, tissu testiculaire de rat, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS, cellules NIH/3T3
• Résultats positifs en FC (Intra): cellules NIH/3T3,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 78 publications below
• IF: See 70 publications below

Informations sur le produit

23418-1-AP cible Osteocalcin dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Osteocalcin Protéine recombinante Ag20065
• Nom complet: bone gamma-carboxyglutamate (gla) protein
• Masse moléculaire calculée: 100 aa, 11 kDa
• Numéro d’acquisition GenBank: BC113432
• Symbole du gène: Osteocalcin
• Identification du gène (NCBI): 632
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Osteocalcin is a small, highly conserved molecule associated with mineralization of bone matrix. Osteocalcin is specifically expressed in osteoblasts and is the most abundant non-collagenous protein in bone. It regulates the dynamics of new bone formation and bone resorption by interaction with vitamin D, and by influencing the differentiation of osteoblasts. Osteocalcin is also involved in the posttranslational targeting of vitamin K-dependent gamma-carboxylation, which controls blood coagulation.

Publications

Species	Application	Title
mouse	IHC	Adv Mater; Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration; Authors - Zhuo Li
human		Bioact Mater; 3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration; Authors - Lei Zhang
mouse	IHC,IF	ACS Nano; Nanotopography Sequentially Mediates Human Mesenchymal Stem Cell-Derived Small Extracellular Vesicles for Enhancing Osteogenesis.; Authors - Liang Ma
mouse	IF	Biomaterials; A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration; Authors - Congcong Yu
mouse	IF	Sci Adv; Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.; Authors - Xiaoqun Li
mouse	IHC	Bone Res; HuR-mediated nucleocytoplasmic translocation of HOTAIR relieves its inhibition of osteogenic differentiation and promotes bone formation; Authors - Yuheng Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Weak bands around the correct size

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,
• Cell Tissue Type: HEART

FH

Jie (Verified Customer) (01-23-2020)

Not working very well in my western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse kidney tissue

FH

Canzhao (Verified Customer) (01-17-2020)

This antibody worked very well on western blot and immunofluorescence, very specific. I recommend this antibody!

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
• Cell Tissue Type: Mouse heart and cultured cardiomyocytes.

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