|Positive WB detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11187-1-AP targets ALPL in WB, IHC, FC, chIP, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||ALPL fusion protein Ag1664|
|Full Name||alkaline phosphatase, liver/bone/kidney|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC021289|
|Gene ID (NCBI)||249|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).This antibody can bind the four mentioned alkaline phosphatases.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
LUNFENG (Verified Customer) (01-27-2020)
Jie (Verified Customer) (01-23-2020)
Smear band but with the right product size on western blot. Immunohistochemical staining showed desired results in control versus knockout mouse model
Jingwen (Verified Customer) (01-17-2020)
This antibody worked very well and showed the specific band around 70kDa in western blot.