Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11187-1-AP targets ALPL in WB, IHC, IF/ICC, FC (Intra), chIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1664 Product name: Recombinant human ALPL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-330 aa of BC021289 Sequence: EKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLL Predict reactive species |
| Full Name | alkaline phosphatase, liver/bone/kidney |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC021289 |
| Gene Symbol | ALPL |
| Gene ID (NCBI) | 249 |
| RRID | AB_2305523 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05186 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is expressed in multiple tissues including liver, bone, and kidney, rather than being restricted to a single tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996). This antibody can bind the four mentioned alkaline phosphatases.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ALPL antibody 11187-1-AP | Download protocol |
| IF protocol for ALPL antibody 11187-1-AP | Download protocol |
| IHC protocol for ALPL antibody 11187-1-AP | Download protocol |
| WB protocol for ALPL antibody 11187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-ALP (catalog no. 11187-1-AP) has accumulated 117 citations published in high-impact journals including Nature Communications, Advanced Science, Biomaterials, ACS Nano, Bone Research, Journal of Experimental & Clinical Cancer Research, Stem Cells, and Journal of Dental Research. Associated research fields span bone biology and osteoporosis, dental and periodontal research, vascular calcification, stem cell biology, tissue engineering and biomaterials, hair regeneration, cancer, neuroinflammation, and liver fibrosis.
| Species | Application | Title |
|---|---|---|
Bioact Mater Metformin carbon dots-based osteogenic and protein delivery system to promote bone regeneration in periodontitis. | ||
Bone Res GsαR201C and estrogen reveal different subsets of bone marrow adiponectin expressing osteogenic cells | ||
Nat Commun Physical and chemical niche of human growth plate for polarized bone development. | ||
Int J Oral Sci VPS4B mutation impairs the osteogenic differentiation of dental follicle cells derived from a patient with dentin dysplasia type I. | ||
ACS Nano Nanoscaled Titanium Oxide Layer Provokes Quick Osseointegration on 3D-Printed Dental Implants: A Domino Effect Induced by Hydrophilic Surface | ||
Gut Microbes Gut dysbiosis-induced vitamin B6 metabolic disorder contributes to chronic stress-related abnormal behaviors in a cortisol-independent manner |
Reviews
What customers say
Users generally rate this antibody 4–5 stars across Western blot, immunofluorescence, and immunohistochemistry applications. In Western blot, it produces a specific band around 70 kDa in mouse heart tissue, though some users report weak signals or background that may be tissue- or cell-line-specific. One user noted a smear band on WB but confirmed the correct molecular weight. For IHC, staining was successful in distinguishing control versus knockout mouse models. Overall, the antibody performs well with minor variability depending on tissue type and experimental conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Charlotte (Verified Customer) (07-25-2024) | I tested the antibody with the following protocol. The most intense band corresponds to ALP. There is a lot of background. Maybe cell line specific as C3H10T1/2 cells are differentiating into the osteoblast lineage upon stimulation ? Blocking 1h in 5% milk in TBST primary incubation 1/1000 in blocking solution, O.N. at 4°C wash 5*5min TBST secondary incubation 1/5000 in blocking solution, 1h at RT° wash 4*5min PBST + 5min PBS
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CHAO (Verified Customer) (03-31-2022) | Weak bands may due to tissue specificity
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LUNFENG (Verified Customer) (01-27-2020) | GOOD
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Jie (Verified Customer) (01-23-2020) | Smear band but with the right product size on western blot. Immunohistochemical staining showed desired results in control versus knockout mouse model
|
Jingwen (Verified Customer) (01-17-2020) | This antibody worked very well and showed the specific band around 70kDa in western blot.
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