MGP Polyclonal antibody

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  IHC staining of human rectal cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse liver using 10734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using MGP antibody (10734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 10734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MGP antibody (10734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse kidney using 10734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MGP antibody (10734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

MGP Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 10734-1-AP

Publications(38)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

GIG36, Cell growth-inhibiting gene 36 protein, matrix Gla protein, MGLAP

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Tested Applications

• Positive IHC detected in: human rectal cancer tissue, human breast cancer tissue, human breast hyperplasia tissue, human colon cancer tissue, human lymphoma tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue, mouse liver tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 10 publications below
• IF: See 8 publications below

Product Information

10734-1-AP targets MGP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MGP fusion protein Ag1091
• Full Name: matrix Gla protein
• Calculated Molecular Weight: 103 aa, 13 kDa
• GenBank Accession Number: BC005272
• Gene Symbol: MGP
• Gene ID (NCBI): 4256
• RRID: AB_2297660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08493
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Matrix Gla protein (MGP), a vitamin K-dependent protein, is recognized as a calcification inhibitor in vascular tissue. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.

Publications

Species	Application	Title
mouse	IHC	Circulation; Bone morphogenic protein antagonists are coexpressed with bone morphogenic protein 4 in endothelial cells exposed to unstable flow in vitro in mouse aortas and in human coronary arteries: role of bone morphogenic protein antagonists in inflammation and atherosclerosis.; Authors - Chang Kyunghwa K
mouse	WB	Nat Commun; Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.; Authors - Mehdi Eshraghi
human	IHC	Arthritis Rheum; Juvenile dermatomyositis calcifications selectively displayed markers of bone formation.; Authors - Urganus Annette L AL
rat	WB	Aging (Albany NY); Intermedin1-53 attenuates aging-associated vascular calcification in rats by upregulating sirtuin 1.; Authors - Yao Chen
human	WB	FASEB J; 2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes injury-induced vascular neointima formation in mice.; Authors - Shumin Guo
human	WB	Int J Obes (Lond); Using gene expression to predict the secretome of differentiating human preadipocytes.; Authors - Mutch D M DM

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (08-29-2023)

This antibody worked pretty well for IF on mouse liver frozen sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Liver