Tested Applications
| Positive IHC detected in | human rectal cancer tissue, human breast cancer tissue, human breast hyperplasia tissue, human colon cancer tissue, human lymphoma tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 25 publications below |
| IHC | See 10 publications below |
| IF | See 9 publications below |
Product Information
10734-1-AP targets MGP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MGP fusion protein Ag1091 Predict reactive species |
| Full Name | matrix Gla protein |
| Calculated Molecular Weight | 103 aa, 13 kDa |
| GenBank Accession Number | BC005272 |
| Gene Symbol | MGP |
| Gene ID (NCBI) | 4256 |
| RRID | AB_2297660 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08493 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Matrix Gla protein (MGP), a vitamin K-dependent protein, is recognized as a calcification inhibitor in vascular tissue. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MGP antibody 10734-1-AP | Download protocol |
| IF protocol for MGP antibody 10734-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Circulation Bone morphogenic protein antagonists are coexpressed with bone morphogenic protein 4 in endothelial cells exposed to unstable flow in vitro in mouse aortas and in human coronary arteries: role of bone morphogenic protein antagonists in inflammation and atherosclerosis. | ||
Nat Commun Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease. | ||
Arthritis Rheum Juvenile dermatomyositis calcifications selectively displayed markers of bone formation. | ||
Aging (Albany NY) Intermedin1-53 attenuates aging-associated vascular calcification in rats by upregulating sirtuin 1. | ||
FASEB J 2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes injury-induced vascular neointima formation in mice. | ||
Int J Obes (Lond) Using gene expression to predict the secretome of differentiating human preadipocytes. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kenzo (Verified Customer) (08-29-2023) | This antibody worked pretty well for IF on mouse liver frozen sections.
|
