Validation Data Gallery
|Positive WB detected in||RAW 264.7 cells, mouse adipose, mouse skeletal muscle, rat heart|
|Positive IHC detected in||human breast cancer tissue, rat brown adipose tissue, human ovary tumor tissue, mouse skin tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:4000|
|Sample-dependent, check data in validation data gallery|
12802-1-AP targets FABP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, geese, hamster, pig, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FABP4 fusion protein Ag3912|
|Full Name||fatty acid binding protein 4, adipocyte|
|Calculated molecular weight||132 aa, 15 kDa|
|Observed molecular weight||15 kDa|
|GenBank accession number||BC003672|
|Gene ID (NCBI)||2167|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.
Cell Death Differ
A novel long noncoding RNA PGC1β-OT1 regulates adipocyte and osteoblast differentiation through antagonizing miR-148a-3p.
J Cell Mol Med
Aspirin inhibits adipogenesis of tendon stem cells and lipids accumulation in rat injury tendon through regulating PTEN/PI3K/AKT signalling.
Biomed Res Int
Significant Role of Dicer and miR-223 in Adipose Tissue of Polycystic Ovary Syndrome Patients.
Front Endocrinol (Lausanne)
A Novel Regulatory Circuit "C/EBPα/miR-20a-5p/TOB2" Regulates Adipogenesis and Lipogenesis.
Biochim Biophys Acta
Nuclear envelope proteins Nesprin2 and LaminA regulate proliferation and apoptosis of vascular endothelial cells in response to shear stress.
Cysteine-rich protein 61 regulates adipocyte differentiation from mesenchymal stem cells through mammalian target of rapamycin complex 1 and canonical Wnt signaling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Huai-Chin (Verified Customer) (05-20-2022)
Western blot on 3T3-L1 cells pre and post adipogenesis. Works pretty well. Strong band in 3T3-L1 cells after adipogenesis.