RUNX2 Polyclonal antibody

RUNX2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Publications

49

Cat no : 20700-1-AP

Synonyms

AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2

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Validation Data Gallery

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  WB analysis of ROS1728 using 20700-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 20700-1-AP (RUNX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20700-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20700-1-AP (RUNX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 20700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20700-1-AP (RUNX2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: ROS1728 cells, MCF-7 cells
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 44 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• ChIP: See 1 publications below

Product Information

20700-1-AP targets RUNX2 in WB, IHC, IF, chIP, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: runt-related transcription factor 2
• Calculated molecular weight: 57 kDa
• Observed molecular weight: 57-60 kDa
• GenBank accession number: NM_004348
• Gene symbol: RUNX2
• Gene ID (NCBI): 860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.

Publications

Species	Application	Title
human	IF,WB	Arterioscler Thromb Vasc Biol; Potential Role of H-Ferritin in Mitigating Valvular Mineralization.; Authors - Katalin Éva Sikura
mouse	WB	J Mech Behav Biomed Mater; Citric acid enhances the physical properties, cytocompatibility and osteogenesis of magnesium calcium phosphate cement.; Authors - Shuang Wang
rat	WB	J Cell Physiol; Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan.; Authors - Yan-Jing Zhang
mouse	WB	BMC Oral Health; ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum.; Authors - Jingyu Wang
human	WB	Ann Transl Med; Inhibition of pyroptosis attenuates Staphylococcus aureus-induced bone injury in traumatic osteomyelitis.; Authors - Xiaobo Zhu
rat	WB	Int J Nanomedicine; The Role of Tantalum Nanoparticles in Bone Regeneration Involves the BMP2/Smad4/Runx2 Signaling Pathway.; Authors - Guilan Zhang

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