RUNX2 Polyclonal antibody
RUNX2 Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IHC, IF, chIP, ELISA
Conjugate
Unconjugated
Cat no : 20700-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | ROS1728 cells, MCF-7 cells |
Positive IF detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 44 publications below |
IHC | See 4 publications below |
IF | See 6 publications below |
ChIP | See 1 publications below |
Product Information
20700-1-AP targets RUNX2 in WB, IHC, IF, chIP, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | runt-related transcription factor 2 |
Calculated molecular weight | 57 kDa |
Observed molecular weight | 57-60 kDa |
GenBank accession number | NM_004348 |
Gene symbol | RUNX2 |
Gene ID (NCBI) | 860 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.
Protocols
Product Specific Protocols | |
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WB protocol for RUNX2 antibody 20700-1-AP | Download protocol |
IF protocol for RUNX2 antibody 20700-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Arterioscler Thromb Vasc Biol Potential Role of H-Ferritin in Mitigating Valvular Mineralization. | ||
J Mech Behav Biomed Mater Citric acid enhances the physical properties, cytocompatibility and osteogenesis of magnesium calcium phosphate cement. | ||
J Cell Physiol Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan. | ||
BMC Oral Health ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum. | ||
Ann Transl Med Inhibition of pyroptosis attenuates Staphylococcus aureus-induced bone injury in traumatic osteomyelitis. | ||
Int J Nanomedicine The Role of Tantalum Nanoparticles in Bone Regeneration Involves the BMP2/Smad4/Runx2 Signaling Pathway. |