Anticorps Polyclonal de lapin anti-IFITM1

• Phare
• Validé par KD/KO

IFITM1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 11727-3-AP

Synonymes

9 27, CD225, IFI17, IFITM1, Interferon induced protein 17, Leu 13 antigen, LEU13

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Galerie de données de validation

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  WB analysis of HepG2 using 11727-3-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11727-3-AP (IFITM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11727-3-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11727-3-AP (IFITM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of ROS1728 using 11727-3-AP

  IP result of anti-IFITM1 (IP:11727-3-AP, 4ug; Detection:11727-3-AP 1:500) with ROS1728 cells lysate 3200ug.

• 

  IHC staining of human lymphoma using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K-562
• Résultats positifs en IP: cellules ROS1728
• Résultats positifs en IHC: tissu de lymphome humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• FC: See 4 publications below

Informations sur le produit

11727-3-AP cible IFITM1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre, Humain, Singe vert d'Afrique
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFITM1 Protéine recombinante Ag2320
• Nom complet: interferon induced transmembrane protein 1 (9-27)
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14-17 kDa
• Numéro d’acquisition GenBank: BC000897
• Symbole du gène: IFITM1
• Identification du gène (NCBI): 8519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFITM1(interferon induced transmembrane protein), also named DSPA2a or interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection.

Publications

Species	Application	Title
human	WB	Nature; Mitotic progression following DNA damage enables pattern recognition within micronuclei.; Authors - Shane M Harding
human	FC	Nature; Intrinsic retroviral reactivation in human preimplantation embryos and pluripotent cells.; Authors - Edward J Grow ; KD Validated
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	WB	Mol Ther; Type 1 Interferon Responses Underlie Tumor-Selective Replication of Oncolytic Measles Virus.; Authors - Sarah Aref
human	WB	Nat Commun; IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses; Authors - M. Clement
human	WB	Cell Rep; IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein.; Authors - Jingyou Yu ; KD Validated

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