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Beta Catenin Polyclonal antibody
Beta Catenin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig and More (6)
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
806
Cat no : 51067-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, human placenta tissue, mouse lung tissue, HeLa cells, SH-SY5Y cells, HEK-293 cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human colon cancer tissue, mouse colon tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | MCF-7 cells, HepG2 cells, HeLa cells, T-47D cells, mouse testis tissue |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51067-2-AP targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, canine, chicken, zebrafish, hamster, sheep, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Calculated Molecular Weight | 781 aa, 86 kDa |
| Observed Molecular Weight | 92 kDa |
| GenBank Accession Number | BC058926 |
| Gene Symbol | Beta Catenin |
| Gene ID (NCBI) | 1499 |
| RRID | AB_2086128 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| IHC protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| IF protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| IP protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells. | ||
Hepatology Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress. | ||
Acta Pharm Sin B Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2. | ||
Hepatology Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma via de-repression of CTNNB1. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Udesh (Verified Customer) (08-16-2023) | Worked well for WB and IF
|
FH Alzbeta (Verified Customer) (03-13-2019) | This antibody was used for IF staining of beta-catenin in primary neonatal rat cardiomyocyte culture. The antibody worked well after both ice cold-methanol or 4% PFA fixation, followed by the 3%BSA block. The incubation with primary (b-catenin) and secondary antibody (aRabbit-Alexa Fluor 488 Plus) lasted 1 hr at room temperature.
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