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  • KD/KO Validated

Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (6)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 51067-2-AP

Synonyms

b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin



Tested Applications

Positive WB detected inA431 cells, mouse lung tissue, human placenta tissue, MCF-7 cells, SH-SY5Y cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue, human breast cancer tissue, mouse colon tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, T-47D cells, mouse testis tissue, HeLa cells, HepG2 cells
Positive FC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

51067-2-AP targets Beta Catenin in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, bovine, canine, chicken, goat, hamster, pig, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC058926
Gene symbol CTNNB1
Gene ID (NCBI) 1499
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Protocols

Product Specific Protocols
WB protocol for Beta Catenin antibody 51067-2-APDownload protocol
IHC protocol for Beta Catenin antibody 51067-2-APDownload protocol
IF protocol for Beta Catenin antibody 51067-2-APDownload protocol
IP protocol for Beta Catenin antibody 51067-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou
humanWB

Hepatology

Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.

Authors - Meng Lu
mouseWB

Hepatology

Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma via de-repression of CTNNB1.

Authors - Sheng-Xian Yuan
humanWB

Nat Commun

CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.

Authors - Yuan Fang
zebrafishIF

Nat Commun

ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.

Authors - Xiaoyun Sun
mouseWB

EMBO Mol Med

Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy.

Authors - Jie-Hong Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alzbeta (Verified Customer) (03-13-2019)

This antibody was used for IF staining of beta-catenin in primary neonatal rat cardiomyocyte culture. The antibody worked well after both ice cold-methanol or 4% PFA fixation, followed by the 3%BSA block. The incubation with primary (b-catenin) and secondary antibody (aRabbit-Alexa Fluor 488 Plus) lasted 1 hr at room temperature.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Primary rat neonatal cardiomyocytes
Beta Catenin Antibody Immunofluorescence, validation (1:100 dilution) in Primary rat neonatal cardiomyocytes (Cat no:51067-2-AP)