- Featured Product
- KD/KO Validated
Beta Catenin Polyclonal antibody
Beta Catenin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 51067-2-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, MCF-7 cells, human placenta tissue, mouse lung tissue, HeLa cells, SH-SY5Y cells, HEK-293 cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue, human liver cancer tissue, mouse colon tissue, mouse liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, T-47D cells, mouse testis tissue, HeLa cells, HepG2 cells|
|Positive FC detected in||MCF-7 cells, HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
51067-2-AP targets Beta Catenin in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat, goat, chicken, sheep, zebrafish, hamster, pig, canine|
|Host / Isotype||Rabbit / IgG|
|Full Name||catenin (cadherin-associated protein), beta 1, 88kDa|
|Calculated molecular weight||781 aa, 86 kDa|
|Observed molecular weight||92 kDa|
|GenBank accession number||BC058926|
|Gene ID (NCBI)||1499|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
|Product Specific Protocols|
|WB protocol for Beta Catenin antibody 51067-2-AP||Download protocol|
|IHC protocol for Beta Catenin antibody 51067-2-AP||Download protocol|
|IF protocol for Beta Catenin antibody 51067-2-AP||Download protocol|
|IP protocol for Beta Catenin antibody 51067-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.
Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.
Acta Pharm Sin B
Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.
Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma via de-repression of CTNNB1.
CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Udesh (Verified Customer) (08-16-2023)
Worked well for WB and IF
Alzbeta (Verified Customer) (03-13-2019)
This antibody was used for IF staining of beta-catenin in primary neonatal rat cardiomyocyte culture. The antibody worked well after both ice cold-methanol or 4% PFA fixation, followed by the 3%BSA block. The incubation with primary (b-catenin) and secondary antibody (aRabbit-Alexa Fluor 488 Plus) lasted 1 hr at room temperature.