IFITM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFITM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

40

Cat no : 12769-1-AP

Synonyms

Interferon-inducible protein 1-8D, Interferon-induced transmembrane protein 2, IFITM 2, DSPA2c, 18D

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Validation Data Gallery

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  WB analysis using 12769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12769-1-AP (IFITM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12769-1-AP (IFITM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12769-1-AP

  WB result of IFITM2 antibody (12769-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFITM2 transfected HepG2 cells.

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  IP experiment of HepG2 using 12769-1-AP

  IP result of anti-IFITM2 (IP:12769-1-AP, 4ug; Detection:12769-1-AP 1:3000) with HepG2 cells lysate 1000 ug.

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  IHC staining of human breast cancer using 12769-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12769-1-AP (IFITM2 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 12769-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12769-1-AP (IFITM2 Antibody) at dilution of 1:50.

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  IHC staining of mouse brain using 12769-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12769-1-AP (IFITM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12769-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12769-1-AP (IFITM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12769-1-AP

  Immunofluorescent analysis of HepG2 cells using 12769-1-AP (IFITM2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HepG2 using 12769-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFITM2 antibody (12769-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, SKOV-3 cells, MCF-7 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human breast cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 33 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• FC: See 4 publications below

Product Information

12769-1-AP targets IFITM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFITM2 fusion protein Ag3451
• Full Name: interferon induced transmembrane protein 2 (1-8D)
• Calculated Molecular Weight: 132 aa, 15 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC009696
• Gene Symbol: IFITM2
• Gene ID (NCBI): 10581
• RRID: AB_2122089
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. Catalog#12769-1-AP is a rabbit polyclonal antibody produced with full-length of human IFITM2.

Publications

Species	Application	Title
human	WB	Cell; The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.; Authors - Abraham L Brass
human	WB	Cell Stem Cell; PMID: 30416070; Authors - Carolina Petrillo
human	WB	Cell Host Microbe; Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.; Authors - Toshana L Foster ; KD Validated
human	WB, FC	Cell Host Microbe; IFITM Proteins Incorporated into HIV-1 Virions Impair Viral Fusion and Spread.; Authors - Alex A Compton ; KD Validated
human	WB	Nat Commun; IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses; Authors - M. Clement
mouse	WB	Nat Commun; BAG3 promotes pancreatic ductal adenocarcinoma growth by activating stromal macrophages.; Authors - Alessandra Rosati ; KD Validated

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