IFITM3 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFITM3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

135

Reviews (2)

Cat no : 11714-1-AP

Synonyms

IP15, Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, IFITM2/3, DSPA2b

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Validation Data Gallery

• 

  WB analysis using 11714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11714-1-AP (IFITM3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11714-1-AP

  IP result of anti-IFITM3 (IP:11714-1-AP, 4ug; Detection:11714-1-AP 1:4000) with HepG2 cells lysate 960 ug.

• 

  IHC staining of human skin cancer using 11714-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 11714-1-AP (IFITM3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFITM3 antibody (11714-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, LNCaP cells, THP-1 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 35 publications below
• WB: See 107 publications below
• IHC: See 9 publications below
• IF: See 32 publications below
• IP: See 6 publications below
• FC: See 8 publications below
• CoIP: See 2 publications below

Product Information

11714-1-AP targets IFITM3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, canine, chicken, goat, african green monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFITM3 fusion protein Ag2285
• Full Name: interferon induced transmembrane protein 3 (1-8U)
• Calculated Molecular Weight: 133 aa, 15 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC006794
• Gene Symbol: IFITM3
• Gene ID (NCBI): 10410
• RRID: AB_2295684
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.

Publications

Species	Application	Title
human	WB	Science; A human apolipoprotein L with detergent-like activity kills intracellular pathogens.; Authors - Ryan G Gaudet
human,mouse	WB	Cell; A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling.; Authors - Noémie Alphonse
human	WB	Nature; IFITM3 restricts the morbidity and mortality associated with influenza.; Authors - Everitt AR
mouse	IHC	Science; T cell memory. Skin-resident memory CD8⁺ T cells trigger a state of tissue-wide pathogen alert.; Authors - Silvia Ariotti
human	IF	Cell Stem Cell; Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids.; Authors - Galina Popova
human	WB,IF	Cell Stem Cell; PMID: 30416070; Authors - Carolina Petrillo ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Thomas (Verified Customer) (06-29-2022)

Works fine in WB and IF

• Applications: Western Blot, Immunofluorescence

FH

Xiuqing (Verified Customer) (03-21-2022)

worked very well for western blot

• Applications: Western Blot
• Cell Tissue Type: MARC-145 cells, H1299 cells

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