|Positive WB detected in||HeLa cells, HepG2 cells, LNCaP cells, THP-1 cells|
|Positive IP detected in||HepG2 cells|
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
11714-1-AP targets IFITM3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, goat, chicken, pig, canine, African green monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFITM3 fusion protein Ag2285|
|Full Name||interferon induced transmembrane protein 3 (1-8U)|
|Calculated molecular weight||133 aa, 15 kDa|
|Observed molecular weight||14 kDa|
|GenBank accession number||BC006794|
|Gene ID (NCBI)||10410|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.
A human apolipoprotein L with detergent-like activity kills intracellular pathogens.
A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling.
IFITM3 restricts the morbidity and mortality associated with influenza.
T cell memory. Skin-resident memory CD8⁺ T cells trigger a state of tissue-wide pathogen alert.
Cell Stem Cell
Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids.
Cell Stem Cell
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Thomas (Verified Customer) (06-29-2022)
Works fine in WB and IF
Xiuqing (Verified Customer) (03-21-2022)
worked very well for western blot