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Caveolin-1 Polyclonal antibody
Caveolin-1 Polyclonal Antibody for IHC, IP, WB,ELISA
Cat no : 16447-1-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, HeLa cells, NIH/3T3 cells|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human breast cancer tissue, human heart tissue, human lung cancer tissue, human liver tissue, human lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 16447-1-AP is Caveolin-1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Caveolin-1 fusion protein Ag8049|
|Full Name||caveolin 1, caveolae protein, 22kDa|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||20-25 kDa|
|GenBank accession number||BC006432|
|Gene ID (NCBI)||857|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in astric Cancer (PMID: 25339030). MW of Caveolin-1 is from 20-25 kDa due to phosphorylation (PMID: 10198051).
RAB31 marks and controls an ESCRT-independent exosome pathway.
Nucleic Acids Res
Nucleosomes enter cells by clathrin- and caveolin-dependent endocytosis.
Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.
Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.
Smart Carbon Nanotubes with Laser-Controlled Behavior in Gene Delivery and Therapy through a Non-Digestive Trafficking Pathway.
Br J Pharmacol
An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (03-15-2022)
Works really well @ 1:1000 for WB in PC3 and DU145 cells. Single band seen.