Validation Data Gallery
|Positive WB detected in||HeLa cells, human brain tissue, MCF-7 cells, PC-3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lung cancer tissue, human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue, HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15981-1-AP targets ISG15 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, goat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ISG15 fusion protein Ag8782|
|Full Name||ISG15 ubiquitin-like modifier|
|Calculated molecular weight||165 aa, 18 kDa|
|Observed molecular weight||15-17 kDa|
|GenBank accession number||BC009507|
|Gene ID (NCBI)||9636|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.
|Product Specific Protocols|
|WB protocol for ISG15 antibody 15981-1-AP||Download protocol|
|IHC protocol for ISG15 antibody 15981-1-AP||Download protocol|
|IF protocol for ISG15 antibody 15981-1-AP||Download protocol|
|IP protocol for ISG15 antibody 15981-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The histone chaperone HIRA promotes the induction of host innate immune defences in response to HSV-1 infection.
E3 ubiquitin ligase RNF6 promotes antiviral immune responses through enhancing the expression of interferon stimulated genes in myeloid cells
ISG15 Connects Autophagy and IFN-γ-Dependent Control of Toxoplasma gondii Infection in Human Cells.
Res Vet Sci
Infection of goats with goatpox virus triggers host antiviral defense through activation of innate immune signaling.
Anti-inflammatory Properties of the Alpha-Melanocyte-Stimulating Hormone in Models of Granulomatous Inflammation.
Pathol Oncol Res
The Overexpression of CD80 and ISG15 Are Associated with the Progression and Metastasis of Breast Cancer by a Meta-Analysis Integrating Three Microarray Datasets.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chaohui (Verified Customer) (10-19-2021)
The antibody shows a high quality of free ISG15 and conjugated ISG15 spots