Validation Data Gallery
|Positive WB detected in
|HeLa cells, human brain tissue, A549 cells, MCF-7 cells, PC-3 cells, IFN alpha treated A459 cells, HepG2 cells, IFN alpha treated HepG2 cells
|Positive IHC detected in
|human lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|mouse brain tissue, HeLa cells
|Western Blot (WB)
|WB : 1:1000-1:4000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15981-1-AP targets ISG15 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, goat, monkey, pig
|Host / Isotype
|Rabbit / IgG
|ISG15 fusion protein Ag8782
|ISG15 ubiquitin-like modifier
|Calculated molecular weight
|165 aa, 18 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.
Signal Transduct Target Ther
TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion
Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease.
ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins.
J Exp Clin Cancer Res
ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma
ISG15 is a critical microenvironmental factor for pancreatic cancer stem cells.
Ribosome biogenesis restricts innate immune responses to virus infection and DNA.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tinne (Verified Customer) (06-27-2023)
This antibody worked really well for detecting an up regulation of ISG15 in IF. Highly recommend!
Chaohui (Verified Customer) (10-19-2021)
The antibody shows a high quality of free ISG15 and conjugated ISG15 spots