IFIT3 Polyclonal antibody

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  WB analysis using 15201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15201-1-AP (IFIT3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 15201-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15201-1-AP (IFIT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 15201-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15201-1-AP (IFIT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

IFIT3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15201-1-AP

Publications(40)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, ChIP, ELISA

Synonyms

CIG 49, CIG49, GARG 49, IFI 60K, IFI60, IFIT 3, IFIT 4, IFIT3, IFIT4, IRG2, ISG 60, ISG60, RIG G

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Tested Applications

• Positive WB detected in: MKN-45 cells, SGC-7901 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 33 publications below
• IHC: See 3 publications below
• IF: See 9 publications below
• ChIP: See 1 publications below

Product Information

15201-1-AP targets IFIT3 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFIT3 fusion protein Ag7349
• Full Name: IFIT3
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC004977
• Gene Symbol: IFIT3
• Gene ID (NCBI): 3437
• RRID: AB_2248738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14879
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human	IF	Adv Sci (Weinh); Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma; Authors - Yongxu Jia
human	WB	Cancer Lett; Induction of IFIT1/IFIT3 and inhibition of Bcl-2 orchestrate the treatment of myeloma and leukemia via pyroptosis; Authors - Yuanming He ; KD Validated
human, mouse	WB	PLoS Pathog; MAVS-MKK7-JNK2 Defines a Novel Apoptotic Signaling Pathway during Viral Infection.; Authors - Yuefeng Huang
human	WB,IF	Front Immunol; ISG15/USP18/STAT2 is a molecular hub regulating IFN I-mediated control of Dengue and Zika virus replication; Authors - Constanza Eleonora Espada
human,mouse	WB	J Neuroinflammation; Progression of pathology in PINK1-deficient mouse brain from splicing via ubiquitination, ER stress, and mitophagy changes to neuroinflammation.; Authors - Sylvia Torres-Odio