|Positive WB detected in||HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15201-1-AP targets IFIT3 in WB, IP, IHC, IF applications and shows reactivity with human samples.
|Cited Reactivity||human, Monkey, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFIT3 fusion protein Ag7349|
|Calculated molecular weight||56 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC004977|
|Gene ID (NCBI)||3437|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.
|Product Specific Protocols|
|WB protocol for IFIT3 antibody 15201-1-AP||Download protocol|
|IHC protocol for IFIT3 antibody 15201-1-AP||Download protocol|
|IF protocol for IFIT3 antibody 15201-1-AP||Download protocol|
|IP protocol for IFIT3 antibody 15201-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mapping the molecular signatures of diet-induced NASH and its regulation by the hepatokine Tsukushi.
Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse.
J Gen Virol
Murine Ifit3 restricts the replication of Rabies virus both in vitro and in vivo.
Poly(I:C) inhibits porcine reproductive and respiratory syndrome virus replication in MARC-145 cells via activation of IFIT3.
Proteomic analysis of the lungs of mice infected with different pathotypes of H5N1 avian influenza viruses.
Mol Cancer Res
STAT1 and NF-κB Inhibitors Diminish Basal Interferon-Stimulated Gene Expression and Improve the Productive Infection of Oncolytic HSV in MPNST Cells.