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  • KD/KO Validated

IFIT1 Polyclonal antibody

IFIT1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23247-1-AP

Synonyms

G10P1, GARG 16, IFI 56, IFI 56K, IFI56, IFIT 1, IFIT1, IFNAI1, ISG56, RNM561



Tested Applications

Positive WB detected inIFN beta treated HeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23247-1-AP is IFIT1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFIT1 fusion protein Ag19691
Full Name interferon-induced protein with tetratricopeptide repeats 1
Calculated molecular weight 478 aa, 55 kDa
Observed molecular weight 55 KDa
GenBank accession numberBC007091
Gene symbol IFIT1
Gene ID (NCBI) 3434
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.

Protocols

Product Specific Protocols
WB protocol for IFIT1 antibody 23247-1-APDownload protocol
IHC protocol for IFIT1 antibody 23247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Neuroinflammation

Progression of pathology in PINK1-deficient mouse brain from splicing via ubiquitination, ER stress, and mitophagy changes to neuroinflammation.

Authors - Sylvia Torres-Odio
humanWB,IHC

Cell Oncol (Dordr)

IFIT1 modulates the proliferation, migration and invasion of pancreatic cancer cells via Wnt/β-catenin signaling.

Authors - Tian-Hao Li
  • KD Validated
humanWB,IHC

Front Oncol

COL8A1 Promotes NSCLC Progression Through IFIT1/IFIT3-Mediated EGFR Activation.

Authors - Xiangyi Zan
WB

J Virol

Herpes Simplex Virus 1 Tegument Protein UL41 Counteracts IFIT3 Antiviral Innate Immunity.

Authors - Zhangtao Jiang
humanWB

J Virol

PMID: 31666385

Authors - Matthew L Scott
humanIF

J Virol

PMID: 31292244

Authors - Gaurav Raikhy