Anticorps Polyclonal de lapin anti-ETS1

• Phare
• Validé par KD/KO

ETS1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 12118-1-AP

Synonymes

ETS 1, ETS1, EWSR2, p54, Protein C ets 1

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Galerie de données de validation

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  WB analysis of Jurkat using 12118-1-AP

  WB result of ETS1 antibody (12118-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ETS1 transfected Jurkat cells.

• 

  WB analysis of Jurkat using 12118-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12118-1-AP (ETS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12118-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12118-1-AP (ETS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12118-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12118-1-AP (ETS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat spleen using 12118-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 12118-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 12118-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 12118-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 12118-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 12118-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 12118-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (12118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules NIH/3T3, tissu de thymus de souris
• Résultats positifs en IHC: tissu splénique de rat, tissu pulmonaire de rat, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IF: See 1 publications below

Informations sur le produit

12118-1-AP cible ETS1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ETS1 Protéine recombinante Ag2127
• Nom complet: v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
• Masse moléculaire calculée: 441 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC017314
• Symbole du gène: ETS1
• Identification du gène (NCBI): 2113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo. The ETS1 protein exists as three isoforms: ETS1-p51, ETS1-p42, ETS1-p27 ranging in size from 27 to 51 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; HIV reprograms host m 6 Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1; Authors - Qiong Zhang
human	WB	Front Cell Dev Biol; rSjP40 Inhibited the Activity of Collagen Type I Promoter via Ets-1 in HSCs.; Authors - Jing Li ; KD Validated
human	WB	Oncotarget; DOT1L promotes angiogenesis through cooperative regulation of VEGFR2 with ETS-1.; Authors - Yang Duan
human	WB	Lung; ETS1 Ameliorates Hyperoxia-Induced Bronchopulmonary Dysplasia in Mice by Activating Nrf2/HO-1 Mediated Ferroptosis; Authors - Min Yang
human	WB	Int J Biol Sci; The RNF26/CBX7 axis modulates the TNF pathway to promote cell proliferation and regulate sensitivity to TKIs in ccRCC.; Authors - Wentao Liu
rat	WB	Commun Biol; The histone demthylase KDM3A protects the myocardium from ischemia/reperfusion injury via promotion of ETS1 expression.; Authors - Xin Guo ; KD Validated

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