EIF4E Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF4E Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 11149-1-AP

Synonyms

mRNA cap-binding protein, eIF-4F 25 kDa subunit, EIF4F, EIF4EL1, eIF-4E

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Validation Data Gallery

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  WB analysis of HEK-293 using 11149-1-AP

  WB result of EIF4E antibody (11149-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF4E transfected HEK-293 cells.

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  WB analysis using 11149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11149-1-AP (EIF4E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11149-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11149-1-AP (EIF4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11149-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11149-1-AP (EIF4E antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 11149-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11149-1-AP (EIF4E antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 11149-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11149-1-AP (EIF4E antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 11149-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF4E antibody (11149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 11149-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug EIF4E Polyclonal antibody (11149-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

11149-1-AP targets EIF4E in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF4E fusion protein Ag1626
• Full Name: eukaryotic translation initiation factor 4E
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 26-29 kDa
• GenBank Accession Number: BC012611
• Gene Symbol: EIF4E
• Gene ID (NCBI): 1977
• RRID: AB_2097686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins. This is a rabbit polyclonal antibody raised against the full-length human EIF4E.

Publications

Species	Application	Title
		Cancer Res; HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.; Authors - Yinghui Li
human	WB	Nucleic Acids Res; SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.; Authors - Yuzhang Du
human	WB	Mol Ther; RNA m6A methylation regulates the dissemination of cancer cells via modulating expression and membrane localization of β-catenin.; Authors - Jiexin Li
human	WB	Theranostics; KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.; Authors - Chengwan Zhang
human	WB	Cell Death Dis; Multifunctional viral protein γ34.5 manipulates nucleolar protein NOP53 for optimal viral replication of HSV-1.; Authors - Wen Meng
mouse	WB	ACS Chem Neurosci; Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus; Authors - Yan Sun