ETS2 Polyclonal antibody

ETS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12280-1-AP

Synonyms

ETS2, ETS2IT1, Protein C ets 2



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, MCF-7 cells, mouse kidney tissue, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse spleen tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12280-1-AP targets ETS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ETS2 fusion protein Ag2929
Full Name v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)
Calculated Molecular Weight 469 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC017040
Gene Symbol ETS2
Gene ID (NCBI) 2114
RRIDAB_10804755
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ETS2 antibody 12280-1-APDownload protocol
IHC protocol for ETS2 antibody 12280-1-APDownload protocol
IP protocol for ETS2 antibody 12280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

The overexpression of Tipe2 in CRC cells suppresses survival while endogenous Tipe2 accelerates AOM/DSS induced-tumor initiation.

Authors - Yan Li
humanWB

Cell Death Dis

A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer

Authors - Yongheng Chen
mouseWB

Gene

Cellular senescence-Related genes define the immune microenvironment and molecular characteristics in severe asthma patients

Authors - Qibin Lin
humanIHC

BMC Med

Single-cell and spatial transcriptomics reveal metastasis mechanism and microenvironment remodeling of lymph node in osteosarcoma

Authors - Yun Liu