FLI1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FLI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11347-1-AP

Synonyms

EWSR2, FLI1, Proto oncogene Fli 1, SIC 1, Transcription factor ERGB

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Validation Data Gallery

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  WB analysis using 11347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11347-1-AP (FLI1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 11347-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11347-1-AP (FLI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11347-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11347-1-AP (FLI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11347-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11347-1-AP (FLI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HL-60 cells, mouse thymus tissue, human brain tissue, Jurkat cells, MOLT-4 cells, Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

11347-1-AP targets FLI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FLI1 fusion protein Ag1899
• Full Name: Friend leukemia virus integration 1
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC010115
• Gene Symbol: FLI1
• Gene ID (NCBI): 2313
• RRID: AB_2106102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing's sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown.

Publications

Species	Application	Title
human,mouse	WB	Mol Ther; Suppression of Fli-1 protects against pericyte loss and cognitive deficits in Alzheimer's disease.; Authors - Pengfei Li
human	IHC	Mol Ther Nucleic Acids; circCAMSAP1 promotes osteosarcoma progression and metastasis by sponging miR-145-5p and regulating FLI1 expression.; Authors - Zizheng Chen
human	WB	Front Cell Infect Microbiol; Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells.; Authors - Fengxue Geng
human	WB	Cancer; Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene.; Authors - Zhang Jianjun J ; KD Validated
human	WB	Mol Med Rep; Fli‑1 promotes metastasis by regulating MMP2 signaling in hepatocellular carcinoma.; Authors - Huiling Wang ; KD Validated