• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-ATF4

ATF4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

N° de cat : 10835-1-AP

Synonymes

TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4



Applications testées

Résultats positifs en WBcellules A431, cellules HEK-293, tissu cérébral de rat
Résultats positifs en IPcellules HEK-293
Résultats positifs en IHCtissu de cancer du sein humain, tissu de côlon de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HeLa traitées à la tunicamycine, cellules HeLa, cellules HepG2
Résultats positifs en FC (Intra)cellules HeLa,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10835-1-AP cible ATF4 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat

Réactivité Humain, rat
Réactivité citéerat, Humain, poisson-zèbre, porc, Hamster
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène ATF4 Protéine recombinante Ag1279
Nom complet activating transcription factor 4 (tax-responsive enhancer element B67)
Masse moléculaire calculée 39 kDa
Poids moléculaire observé45-50 kDa
Numéro d’acquisition GenBankBC022088
Symbole du gène ATF4
Identification du gène (NCBI) 468
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of ATF4?

The molecular weight of ATF is 38.6 kD.

 

What is ATF4?

Activating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor (PMIDs: 16000305, 17485283).

 

What the function of ATF4?

ATF4 its a transcription factor that controls the transcriptional activity of mature osteoblasts. ATF4 is particularly critical for their timely onset and terminal differentiation, as well as expression of Bsp and osteocalcin. Knockout animals displayed reduction or delay in bone mineralization and have severely reduced bone volume. ATF4 is also part of the PERK-eIF2α-ATF4-CHOP apoptosis pathway, which is activated by ER stress, and it likely plays a role related to tumor cell survival (PMIDs: 18083928, 16000305, 30134550).

 

What is the effect of ATF4 interaction with RSK2?

ATF4 and RSK2 posttranscriptionally regulate type I collagen synthesis. Lack of RSK2 phosphorylation of AFT4 may contribute to skeletal phenotypes associated with Coffin-Lowry Syndrome (PMID: 17485283).

 

Where is ATF4 expressed?

ATF4 protein is predominantly expressed in osteoblasts, although its corresponding Atf4 mRNA is ubiquitously expressed (PMID: 16000305).

 

What regulates ATF4 expression?

ATF4 is regulated by a ubiquitin/proteasomal pathway, which is less active in osteoblasts by inhibition with MG115 (PMID: 16000305).

 

How does ATF4 expression affect Ocn mRNA?

Inhibition of the degradation pathway leads to ATF4 accumulation and induces Ocn mRNA expression in non-osteoblastic cells (PMID: 16000305).

 

Does ATF4 have the ability to induce osteoblast-specific gene expression even in non-osteoblastic cells?

Yes, ATF4, as well as other osteoblast differentiation factors, has this ability. AFT4 interactions with Runx2 can stimulate osteoblast-specific osteocalcin gene expression. (PMIDs: 16000305, 17485283)


Protocole

Product Specific Protocols
WB protocol for ATF4 antibody 10835-1-APDownload protocol
IHC protocol for ATF4 antibody 10835-1-APDownload protocol
IF protocol for ATF4 antibody 10835-1-APDownload protocol
IP protocol for ATF4 antibody 10835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell

Large neutral amino acid levels tune perinatal neuronal excitability and survival

Authors - Lisa S Knaus
humanWB

Cell

Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output.

Authors - Qiyi Zhao
humanWB

Cell

Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis.

Authors - Tslil Ast
WB

Cell Metab

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Authors - Hui Luo
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
humanWB

Nucleic Acids Res

Translation regulation of specific mRNAs by RPS26 C-terminal RNA-binding tail integrates energy metabolism and AMPK-mTOR signaling

Authors - Tal Havkin-Solomon

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xu-Qiao (Verified Customer) (10-24-2023)

Although there is a band at 50 kd, many other non-specific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain lysate
ATF4 Antibody Western Blot validation (1:1000 dilution) in mouse brain lysate (Cat no:10835-1-AP)
FH

Lucile (Verified Customer) (08-01-2023)

The ATF4 antibody worked well for immunoprecipitation, western blotting (1:1000 but based on initial results could dilute further) and gave a very bright IF signal on fixed smooth muscle cells.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
FH

SU (Verified Customer) (03-22-2022)

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
ATF4 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:10835-1-AP)
FH

Natalie (Verified Customer) (12-11-2018)

Antibody worked well, although does detect some non-specific bands which appear to be dimers from the molecular weight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:750
  • Cell Tissue Type: Rat DRG & Sciatic nerve