Anticorps Polyclonal de lapin anti-ATF4

• Phare
• Validé par KD/KO

ATF4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

380

Avis (4)

N° de cat : 10835-1-AP

Synonymes

TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4

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Galerie de données de validation

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  WB analysis of A431 using 10835-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10835-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10835-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10835-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10835-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10835-1-AP

  IP result of anti-ATF4 (IP:10835-1-AP, 3ug; Detection:10835-1-AP 1:400) with HEK-293 cells lysate 6000ug.

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  IHC staining of human breast cancer using 10835-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10835-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10835-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10835-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10835-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10835-1-AP (ATF4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10835-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using ATF4 antibody (10835-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 10835-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ATF4 (10835-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, tissu cérébral de rat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa traitées à la tunicamycine, cellules HeLa, cellules HepG2
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 23 publications below
• WB: See 355 publications below
• IHC: See 29 publications below
• IF: See 24 publications below
• IP: See 4 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 4 publications below

Informations sur le produit

10835-1-AP cible ATF4 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, poisson-zèbre, porc, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATF4 Protéine recombinante Ag1279
• Nom complet: activating transcription factor 4 (tax-responsive enhancer element B67)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC022088
• Symbole du gène: ATF4
• Identification du gène (NCBI): 468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of ATF4?

The molecular weight of ATF is 38.6 kD.

What is ATF4?

Activating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor (PMIDs: 16000305, 17485283).

What the function of ATF4?

ATF4 its a transcription factor that controls the transcriptional activity of mature osteoblasts. ATF4 is particularly critical for their timely onset and terminal differentiation, as well as expression of Bsp and osteocalcin. Knockout animals displayed reduction or delay in bone mineralization and have severely reduced bone volume. ATF4 is also part of the PERK-eIF2α-ATF4-CHOP apoptosis pathway, which is activated by ER stress, and it likely plays a role related to tumor cell survival (PMIDs: 18083928, 16000305, 30134550).

What is the effect of ATF4 interaction with RSK2?

ATF4 and RSK2 posttranscriptionally regulate type I collagen synthesis. Lack of RSK2 phosphorylation of AFT4 may contribute to skeletal phenotypes associated with Coffin-Lowry Syndrome (PMID: 17485283).

Where is ATF4 expressed?

ATF4 protein is predominantly expressed in osteoblasts, although its corresponding Atf4 mRNA is ubiquitously expressed (PMID: 16000305).

What regulates ATF4 expression?

ATF4 is regulated by a ubiquitin/proteasomal pathway, which is less active in osteoblasts by inhibition with MG115 (PMID: 16000305).

How does ATF4 expression affect Ocn mRNA?

Inhibition of the degradation pathway leads to ATF4 accumulation and induces Ocn mRNA expression in non-osteoblastic cells (PMID: 16000305).

Does ATF4 have the ability to induce osteoblast-specific gene expression even in non-osteoblastic cells?

Yes, ATF4, as well as other osteoblast differentiation factors, has this ability. AFT4 interactions with Runx2 can stimulate osteoblast-specific osteocalcin gene expression. (PMIDs: 16000305, 17485283)

Publications

Species	Application	Title
	WB	Cell; Large neutral amino acid levels tune perinatal neuronal excitability and survival; Authors - Lisa S Knaus
human	WB	Cell; Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output.; Authors - Qiyi Zhao
human	WB	Cell; Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis.; Authors - Tslil Ast
	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Nucleic Acids Res; Translation regulation of specific mRNAs by RPS26 C-terminal RNA-binding tail integrates energy metabolism and AMPK-mTOR signaling; Authors - Tal Havkin-Solomon

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xu-Qiao (Verified Customer) (10-24-2023)

Although there is a band at 50 kd, many other non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain lysate

ATF4 Antibody Western Blot validation (1:1000 dilution) in mouse brain lysate (Cat no:10835-1-AP)

FH

Lucile (Verified Customer) (08-01-2023)

The ATF4 antibody worked well for immunoprecipitation, western blotting (1:1000 but based on initial results could dilute further) and gave a very bright IF signal on fixed smooth muscle cells.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:1000

FH

SU (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: H9C2

ATF4 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:10835-1-AP)

FH

Natalie (Verified Customer) (12-11-2018)

Antibody worked well, although does detect some non-specific bands which appear to be dimers from the molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:750
• Cell Tissue Type: Rat DRG & Sciatic nerve

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