- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-ATF4
ATF4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat et plus (3)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA
Conjugaison
Non conjugué
380
N° de cat : 10835-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules A431, cellules HEK-293, tissu cérébral de rat |
Résultats positifs en IP | cellules HEK-293 |
Résultats positifs en IHC | tissu de cancer du sein humain, tissu de côlon de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HeLa traitées à la tunicamycine, cellules HeLa, cellules HepG2 |
Résultats positifs en FC (Intra) | cellules HeLa, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
10835-1-AP cible ATF4 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat
Réactivité | Humain, rat |
Réactivité citée | rat, Humain, poisson-zèbre, porc, Hamster |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | ATF4 Protéine recombinante Ag1279 |
Nom complet | activating transcription factor 4 (tax-responsive enhancer element B67) |
Masse moléculaire calculée | 39 kDa |
Poids moléculaire observé | 45-50 kDa |
Numéro d’acquisition GenBank | BC022088 |
Symbole du gène | ATF4 |
Identification du gène (NCBI) | 468 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
What is the molecular weight of ATF4?
The molecular weight of ATF is 38.6 kD.
What is ATF4?
Activating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor (PMIDs: 16000305, 17485283).
What the function of ATF4?
ATF4 its a transcription factor that controls the transcriptional activity of mature osteoblasts. ATF4 is particularly critical for their timely onset and terminal differentiation, as well as expression of Bsp and osteocalcin. Knockout animals displayed reduction or delay in bone mineralization and have severely reduced bone volume. ATF4 is also part of the PERK-eIF2α-ATF4-CHOP apoptosis pathway, which is activated by ER stress, and it likely plays a role related to tumor cell survival (PMIDs: 18083928, 16000305, 30134550).
What is the effect of ATF4 interaction with RSK2?
ATF4 and RSK2 posttranscriptionally regulate type I collagen synthesis. Lack of RSK2 phosphorylation of AFT4 may contribute to skeletal phenotypes associated with Coffin-Lowry Syndrome (PMID: 17485283).
Where is ATF4 expressed?
ATF4 protein is predominantly expressed in osteoblasts, although its corresponding Atf4 mRNA is ubiquitously expressed (PMID: 16000305).
What regulates ATF4 expression?
ATF4 is regulated by a ubiquitin/proteasomal pathway, which is less active in osteoblasts by inhibition with MG115 (PMID: 16000305).
How does ATF4 expression affect Ocn mRNA?
Inhibition of the degradation pathway leads to ATF4 accumulation and induces Ocn mRNA expression in non-osteoblastic cells (PMID: 16000305).
Does ATF4 have the ability to induce osteoblast-specific gene expression even in non-osteoblastic cells?
Yes, ATF4, as well as other osteoblast differentiation factors, has this ability. AFT4 interactions with Runx2 can stimulate osteoblast-specific osteocalcin gene expression. (PMIDs: 16000305, 17485283)
Protocole
Product Specific Protocols | |
---|---|
WB protocol for ATF4 antibody 10835-1-AP | Download protocol |
IHC protocol for ATF4 antibody 10835-1-AP | Download protocol |
IF protocol for ATF4 antibody 10835-1-AP | Download protocol |
IP protocol for ATF4 antibody 10835-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output. | ||
Cell Metab AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity. | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. | ||
Nucleic Acids Res Translation regulation of specific mRNAs by RPS26 C-terminal RNA-binding tail integrates energy metabolism and AMPK-mTOR signaling |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Xu-Qiao (Verified Customer) (10-24-2023) | Although there is a band at 50 kd, many other non-specific bands.
|
FH Lucile (Verified Customer) (08-01-2023) | The ATF4 antibody worked well for immunoprecipitation, western blotting (1:1000 but based on initial results could dilute further) and gave a very bright IF signal on fixed smooth muscle cells.
|
FH SU (Verified Customer) (03-22-2022) |
|
FH Natalie (Verified Customer) (12-11-2018) | Antibody worked well, although does detect some non-specific bands which appear to be dimers from the molecular weight.
|