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CHOP; GADD153 Polyclonal antibody
CHOP; GADD153 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 15204-1-AP
Validation Data Gallery
|Positive WB detected in||Tunicamycin treated HeLa cells, HeLa cells, MCF-7 cells, K-562 cells, C6 cells, RAW 264.7 cells, HSC-T6 cells, NIH/3T3 cells|
|Positive IHC detected in||human colon cancer tissue, human cervical cancer tissue, human breast cancer tissue, human thyroid cancer tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Tunicamycin treated HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
15204-1-AP targets CHOP; GADD153 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, zebrafish, Hamster, pig, Bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHOP; GADD153 fusion protein Ag7354|
|Full Name||DNA-damage-inducible transcript 3|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC003637|
|Gene ID (NCBI)||1649|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Fan (Verified Customer) (03-22-2021)
Mouse retinal tissue was used. Single band detected at the right size.
Daniel (Verified Customer) (06-25-2019)
Specific staining detected in the proximal tubules.