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CHOP; GADD153 Polyclonal antibody
CHOP; GADD153 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA
Conjugate
Unconjugated
423
Cat no : 15204-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Tunicamycin treated HeLa cells, HeLa cells, MCF-7 cells, K-562 cells, C6 cells, RAW 264.7 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue, human breast cancer tissue, human thyroid cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Tunicamycin treated HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 20 publications below |
WB | See 389 publications below |
IHC | See 43 publications below |
IF | See 38 publications below |
IP | See 3 publications below |
CoIP | See 1 publications below |
ChIP | See 1 publications below |
Product Information
15204-1-AP targets CHOP; GADD153 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, zebrafish, Hamster, pig, Bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CHOP; GADD153 fusion protein Ag7354 |
Full Name | DNA-damage-inducible transcript 3 |
Calculated molecular weight | 19 kDa |
Observed molecular weight | 30 kDa |
GenBank accession number | BC003637 |
Gene symbol | DDIT3 |
Gene ID (NCBI) | 1649 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CHOP; GADD153 antibody 15204-1-AP | Download protocol |
IHC protocol for CHOP; GADD153 antibody 15204-1-AP | Download protocol |
IF protocol for CHOP; GADD153 antibody 15204-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. | ||
Mol Cell The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma. | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. | ||
Cell Death Differ Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation | ||
Nat Commun Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Fan (Verified Customer) (03-22-2021) | Mouse retinal tissue was used. Single band detected at the right size.
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FH Daniel (Verified Customer) (06-25-2019) | Specific staining detected in the proximal tubules.
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