CHOP; GADD153 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHOP; GADD153 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

423

Reviews (2)

Cat no : 15204-1-AP

Synonyms

C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP; GADD153, CHOP10, DDIT 3, DDIT3, GADD153

---

Validation Data Gallery

• 

  WB analysis of HeLa using 15204-1-AP

  WB result of CHOP antibody (15204-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHOP transfected HeLa cells.

• 

  WB analysis using 15204-1-AP

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15204-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15204-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using CHOP; GADD153 antibody (15204-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 15204-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CHOP; GADD153 (15204-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

• 

  FC experiment of HeLa using 15204-1-AP

  1X10^6 HeLa cells untreated (dashed lines) or treated with Tunicamycin (20 μg/mL, 4 h; red) were intracellularly stained with 0.4 ug Anti-Human CHOP; GADD153 (15204-1-AP) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

---

Tested Applications

• Positive WB detected in: Tunicamycin treated HeLa cells, HeLa cells, MCF-7 cells, K-562 cells, C6 cells, RAW 264.7 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue, human cervical cancer tissue, human breast cancer tissue, human thyroid cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Tunicamycin treated HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 20 publications below
• WB: See 389 publications below
• IHC: See 43 publications below
• IF: See 38 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

15204-1-AP targets CHOP; GADD153 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, zebrafish, Hamster, pig, Bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHOP; GADD153 fusion protein Ag7354
• Full Name: DNA-damage-inducible transcript 3
• Calculated molecular weight: 19 kDa
• Observed molecular weight: 30 kDa
• GenBank accession number: BC003637
• Gene symbol: DDIT3
• Gene ID (NCBI): 1649
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Publications

Species	Application	Title
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
mouse	IF	Acta Neuropathol; Wolframin is a novel regulator of tau pathology and neurodegeneration.; Authors - Shuo Chen
human	WB,IHC,IP,ChIP	Mol Cell; The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma.; Authors - Hayley J Zullow
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
mouse,rat	WB	Cell Death Differ; Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation; Authors - Guangjin Chen
mouse	WB	Nat Commun; Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation.; Authors - Feixiang Yuan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Fan (Verified Customer) (03-22-2021)

Mouse retinal tissue was used. Single band detected at the right size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

CHOP; GADD153 Antibody Western Blot validation (1:500 dilution) in (Cat no:15204-1-AP)

FH

Daniel (Verified Customer) (06-25-2019)

Specific staining detected in the proximal tubules.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse kidney (TRACK model)

CHOP; GADD153 Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse kidney (TRACK model) (Cat no:15204-1-AP)

Related Products

FUS/TLS Polyclonal antibody

11570-1-AP

human BCL2 Polyclonal antibody

12789-1-AP

EIF2A/CDA02 Polyclonal antibody

11233-1-AP

AKT Polyclonal antibody

10176-2-AP

ATF4 Polyclonal antibody

10835-1-AP

BAX Polyclonal antibody

50599-2-Ig

Caspase 3/p17/p19 Polyclonal antibody

19677-1-AP

ATF6 Polyclonal antibody

24169-1-AP