CHOP/GADD153 Polyclonal antibody

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  WB analysis of HeLa using 15204-1-AP

  WB result of CHOP antibody (15204-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHOP transfected HeLa cells.

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  WB analysis using 15204-1-AP

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15204-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of C6 using 15204-1-AP

  IP result of anti-CHOP; GADD153 (IP:15204-1-AP, 4ug; Detection:15204-1-AP 1:4000) with C6 cells lysate 1600 ug.

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  IHC staining of human breast cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15204-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using CHOP; GADD153 antibody (15204-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HeLa using 15204-1-AP

  1X10^6 HeLa cells untreated (dashed lines) or treated with Tunicamycin (20 μg/mL, 4 h; red) were intracellularly stained with 0.4 ug Anti-Human CHOP; GADD153 (15204-1-AP) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• Featured Product
• KD/KO Validated

CHOP/GADD153 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15204-1-AP

Publications(599)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

CHOP; GADD153, CHOP, DDIT3, GADD153, CCAAT/enhancer-binding protein homologous protein

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Tested Applications

• Positive WB detected in: Tunicamycin treated HeLa cells, MCF-7 cells, HeLa cells, K-562 cells, RAW 264.7 cells
• Positive IP detected in: C6 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human thyroid cancer tissue, human cervical cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Tunicamycin treated HeLa cells
• Positive FC (Intra) detected in: Tunicamycin treated HeLa cells

Samples need to be treated with ER stress.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 23 publications below
• WB: See 552 publications below
• IHC: See 67 publications below
• IF: See 67 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Product Information

15204-1-AP targets CHOP/GADD153 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, canine, chicken, zebrafish, bovine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHOP/GADD153 fusion protein Ag7354
• Full Name: DNA-damage-inducible transcript 3
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC003637
• Gene Symbol: CHOP
• Gene ID (NCBI): 1649
• RRID: AB_2292610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35638
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Publications

Species	Application	Title
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu
mouse	IF	Acta Neuropathol; Wolframin is a novel regulator of tau pathology and neurodegeneration.; Authors - Shuo Chen
human	WB,IHC,IP,ChIP	Mol Cell; The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma.; Authors - Hayley J Zullow
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Cell Rep Med; Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition; Authors - Yu Chang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fan (Verified Customer) (03-22-2021)

Mouse retinal tissue was used. Single band detected at the right size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

CHOP/GADD153 Antibody Western Blot validation (1:500 dilution) in (Cat no:15204-1-AP)

FH

Daniel (Verified Customer) (06-25-2019)

Specific staining detected in the proximal tubules.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse kidney (TRACK model)

CHOP/GADD153 Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse kidney (TRACK model) (Cat no:15204-1-AP)