ATF2 Polyclonal antibody

ATF2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11908-1-AP

Synonyms

CREB-2, CREB2, CREB 2, cAMP-responsive element-binding protein 2, cAMP-dependent transcription factor ATF-2

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Validation Data Gallery

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  WB analysis using 11908-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11908-1-AP (ATF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11908-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11908-1-AP (ATF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11908-1-AP

  IP result of anti-ATF2 (IP:11908-1-AP, 4ug; Detection:11908-1-AP 1:300) with mouse brain tissue lysate 3200 ug.

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  IF Staining of HeLa using 11908-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11908-1-AP (ATF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse brain tissue, HeLa cells, K-562 cells
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11908-1-AP targets ATF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATF2 fusion protein Ag2581
• Full Name: activating transcription factor 2
• Calculated Molecular Weight: 209 aa, 23 kDa
• Observed Molecular Weight: 60 kDa, 64 kDa
• GenBank Accession Number: BC026175
• Gene Symbol: ATF2
• Gene ID (NCBI): 1386
• RRID: AB_10695892
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody can recognize all the isoforms of human ATF2. The molecular weight detected is larger than the theoretical value, which may be related to protein modification. About 65-68 kDa band detected in the articles(PMID: 19135136; PMID: 28628081 ).

Publications

Species	Application	Title
human	WB	Mar Drugs; 7-Acetylsinumaximol B Induces Apoptosis and Autophagy in Human Gastric Carcinoma Cells through Mitochondria Dysfunction and Activation of the PERK/eIF2α/ATF4/CHOP Signaling Pathway.; Authors - Tsung-Chang Tsai
human	WB,IF	Transl Cancer Res; Annexin A7 and JNK knockdown suppress the lymphatic metastasis potential of hepatocellular carcinoma cells: Possible contributions of ATF2 and sequence-related lncRNA NONMMUT114121.1.; Authors - Qi Deng
rat	WB	Korean J Physiol Pharmacol; The WNT/Ca2+ pathway promotes atrial natriuretic peptide secretion by activating protein kinase C/transforming growth factor-β activated kinase 1/activating transcription factor 2 signaling in isolated beating rat atria; Authors - Zhi-yu Li
mouse	WB,IHC	J Ethnopharmacol; Isoastragaloside I attenuates cholestatic liver diseases by ameliorating liver injury, regulating bile acid metabolism and restoring intestinal barrier; Authors - Linzhang Zhang